Agrigenetics Advanced Research Division, 5649 East Buckeye Road, 53716, Madison, WI, U.S.A..
Plant Mol Biol. 1984 Mar;3(2):111-7. doi: 10.1007/BF00040035.
Oligodeoxynucleotides complementary to the deduced mRNA sequence of soybean Kunitz trypsin inhibitor (KTI) were used to prime the synthesis of cDNA from soybean cotyledon total poly(A) RNA. The primed cDNA was used to select clones from a Glycine max cotyledon cDNA library. Two out of twelve hybridizing clones were shown to contain KTI cDNA. The nucleotide sequence of one clone, pSTI 9-2, was determined and it was found to encompass the complete protein coding region of KTI excet for three C-terminal residues. Trypsin inhibitor is synthesized with a 25 amino acid hydrophobic N-terminal sequence presumed to be a signal peptide. The mature polypeptide encoded by pSTI 9-2 agrees with the published amino acid composition of KTI, but contains two discrepancies at the peptide sequence level.
寡聚脱氧核苷酸与大豆 Kunitz 胰蛋白酶抑制剂（KTI）的推导 mRNA 序列互补，用于从大豆子叶总 poly(A)RNA 中启动 cDNA 的合成。用启动的 cDNA 从大豆子叶 cDNA 文库中筛选克隆。在 12 个杂交克隆中有 2 个被证明含有 KTI cDNA。克隆 pSTI 9-2 的核苷酸序列被确定，发现它包含了 KTI 的完整蛋白编码区，除了 3 个 C 末端残基。胰蛋白酶抑制剂合成时有一个 25 个氨基酸的疏水 N 端序列，推测是一个信号肽。pSTI 9-2 编码的成熟多肽与已发表的 KTI 的氨基酸组成一致，但在肽序列水平上有两个差异。
