多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
作者信息
Ito H, Ike Y, Ikuta S, Itakura K
出版信息
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
A simple solid phase method for the synthesis of oligodeoxyribonucleotides has been developed using the phosphotriester approach. Mononucleotide coupling units are sequentially added to the polystyrene copolymer with 1% divinylbenzene and two kinds of oligonucleotides, d(CACGACCCCTCCACGT) and d(AACTGGTATTACTGGGCG), are synthesized in a relatively high yield. One cycle of the mononucleotide addition is about 70 minutes, and this method is particularly suitable for the automation of the synthesis upon availability of an automatic synthesizer.
已开发出一种使用磷酸三酯法合成寡脱氧核糖核苷酸的简单固相方法。将单核苷酸偶联单元依次添加到含有1%二乙烯基苯的聚苯乙烯共聚物上，并以相对较高的产率合成了两种寡核苷酸，即d(CACGACCCCTCCACGT)和d(AACTGGTATTACTGGGCG)。单核苷酸添加的一个循环约为70分钟，并且一旦有自动合成仪，该方法特别适合于合成的自动化。
Zotero 插件