Boll Weevil Research Lab., Agricultural Research Service USDA, P.O. Box 5367, 39762, Mississippi State, Mississippi.
J Chem Ecol. 1985 Sep;11(9):1229-38. doi: 10.1007/BF01024111.
Column chromatography of the pentane extract of freeze-dried cotton buds or anthers yielded a wax-sterol ester fraction that exhibited potent feeding stimulant activity for the cotton boll weevil. The waxes of the wax-sterol ester mixture were responsible for the feeding activity. Saponification of the wax-sterol ester fraction yielded about 15% alcohols and 85% sterols. A C18∶1 alcohol, dihydrophytol, phytol, and geranylgeraniol constituted 15, 36, 26, and 23%, respectively, of the total alcohols, implicating certain of their long-chain esters as feeding stimulants. Several esters of dihydrophytol, phytol, and geranylgeraniol were identified among the waxes by GC-MS. Certain phytol, geranylgeraniol, and oleyl alcohol esters containing C12 to C26 acid moieties were synthesized and were found to induce high feeding stimulant activity in the cotton boll weevil.
柱层析法分离冻干棉铃或花药的戊烷提取物，得到一种蜡质-甾醇酯馏分，对棉铃象鼻虫具有强烈的取食刺激活性。蜡质-甾醇酯混合物中的蜡质是取食活性的原因。蜡质-甾醇酯馏分的皂化得到约 15%的醇和 85%的甾醇。C18∶1 醇、二氢植醇、植醇和香叶基香叶醇分别占总醇的 15%、36%、26%和 23%，表明它们的某些长链酯类是取食刺激物。通过 GC-MS 在蜡质中鉴定出几种二氢植醇、植醇和香叶基香叶醇的酯类。合成了含有 C12 至 C26 酸部分的某些植醇、香叶基香叶醇和油醇酯类，并发现它们对棉铃象鼻虫具有很高的取食刺激活性。
