Gellerman J L, Anderson W H, Schlenk H
Lipids. 1975 Nov;10(11):656-61. doi: 10.1007/BF02532757.
An efficient procedure for preparing phytenic acid methyl ester, free of isomers, from phytol is reported. Phytyl phytenate and other isoprenoid wax esters were synthesized. Gas liquid chromatography of these wax esters and other compounds related to phytol and phytenic acid is described. The alkyl constituents of isoprenoid wax esters can be analyzed after alkaline methanolysis and the acyl constituents after acidic methanolysis. The applicability of these methods to natural mixtures was demonstrated with wax esters from mosses which contained both types of isoprenoids and with wax esters from healthy and frost damaged grass which contained phytol, but not phytenic acid.
报道了一种从叶绿醇制备无异构体的植酸甲酯的有效方法。合成了植酸植醇酯和其他类异戊二烯蜡酯。描述了这些蜡酯以及与叶绿醇和植酸相关的其他化合物的气相色谱分析。类异戊二烯蜡酯的烷基成分可在碱性甲醇解后进行分析，酰基成分则在酸性甲醇解后进行分析。这些方法对天然混合物的适用性通过来自含有两种类异戊二烯的苔藓蜡酯以及来自含有叶绿醇但不含植酸的健康和受冻害草的蜡酯得到了证明。
