Chiplunkar S, Langhorne J, Kaufmann S H
J Immunol. 1986 Dec 15;137(12):3748-52.
Purified splenic B cells from C57BL/6 mice were separated into high-density (resting) and low-density (activated) B cells. Separated B cell populations were cultured at low cell densities (1 X 10(4) cells/well) with recombinant interleukin 1 (r-IL 1) alone or in combination with dextran sulfate (DXS) or anti-IgM monoclonal antibodies (alpha IgM mab), respectively, and proliferative responses were determined. R-IL 1 alone, as well as in synergy with alpha IgM mab or DXS, respectively, stimulated the growth of low-density B cells. Moreover, r-IL 1 and alpha IgM mab costimulated replication of high-density B cells. Separated B cell populations (1 X 10(5) cells/well) were cultured with r-IL 1 alone or in combination with DXS or alpha IgM mab, respectively, and the generation of plaque-forming cells was determined. R-IL 1 alone, as well as in synergy with DXS, stimulated the differentiation of low-density B cells into Ig-secreting cells. These findings suggest that r-IL 1 has B cell growth and differentiation factor activity and is operative on high- and low-density B cells. Thus, IL 1 may play an important role in B cell growth and maturation.
从C57BL/6小鼠中分离出的纯化脾B细胞被分为高密度（静止）和低密度（活化）B细胞。将分离出的B细胞群体以低细胞密度（1×10⁴个细胞/孔）分别与重组白细胞介素1（r-IL 1）单独培养，或与硫酸葡聚糖（DXS）或抗IgM单克隆抗体（α IgM mab）联合培养，并测定增殖反应。单独的r-IL 1以及分别与α IgM mab或DXS协同作用时，均可刺激低密度B细胞的生长。此外，r-IL 1和α IgM mab共同刺激高密度B细胞的增殖。将分离出的B细胞群体（1×10⁵个细胞/孔）分别与r-IL 1单独培养，或与DXS或α IgM mab联合培养，并测定空斑形成细胞的产生。单独的r-IL 1以及与DXS协同作用时，均可刺激低密度B细胞分化为分泌Ig的细胞。这些发现表明，r-IL 1具有B细胞生长和分化因子活性，并且对高密度和低密度B细胞均起作用。因此，IL 1可能在B细胞生长和成熟中发挥重要作用。
