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从土壤中分离和鉴定链霉菌物种作为防治真菌植物病原菌的潜在生物防治剂。

Isolation and characterization of soil Streptomyces species as potential biological control agents against fungal plant pathogens.

机构信息

Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Unidad Sureste, Calle 30 No. 151 (7 y 7A). Col. García Ginerés, C.P. 97070, Mérida, Yucatán, México,

出版信息

World J Microbiol Biotechnol. 2014 May;30(5):1639-47. doi: 10.1007/s11274-013-1568-x. Epub 2013 Dec 6.

Abstract

The use of antagonist microorganisms against fungal plant pathogens is an attractive and ecologically alternative to the use of chemical pesticides. Streptomyces are beneficial soil bacteria and potential candidates for biocontrol agents. This study reports the isolation, characterization and antagonist activity of soil streptomycetes from the Los Petenes Biosphere Reserve, a Natural protected area in Campeche, Mexico. The results showed morphological, physiological and biochemical characterization of six actinomycetes and their inhibitory activity against Curvularia sp., Aspergillus niger, Helminthosporium sp. and Fusarium sp. One isolate, identified as Streptomyces sp. CACIS-1.16CA showed the potential to inhibit additional pathogens as Alternaria sp., Phytophthora capsici, Colletotrichum sp. and Rhizoctonia sp. with percentages ranging from 47 to 90 %. This study identified a streptomycete strain with a broad antagonist activity that could be used for biocontrol of plant pathogenic fungi.

摘要

利用拮抗微生物来防治植物病原真菌是一种有吸引力的、生态替代化学农药的方法。链霉菌是有益的土壤细菌,也是生物防治剂的潜在候选者。本研究报告了来自墨西哥坎佩切洛斯彼德内斯生物圈保护区的土壤链霉菌的分离、鉴定和拮抗活性。结果表明,六种放线菌具有形态学、生理学和生物化学特征,并且对旋孢腔菌、黑曲霉、腐霉和镰刀菌具有抑制活性。一种被鉴定为链霉菌的菌株 CACIS-1.16CA 表现出抑制其他病原体的潜力,如链格孢菌、辣椒疫霉、炭疽菌和立枯丝核菌,抑制率为 47%至 90%。本研究鉴定出一种具有广泛拮抗活性的链霉菌菌株,可用于防治植物病原真菌。

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