• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

土著马铃薯（Solanum tuberosum subsp. tuberosum L.）内生放线菌对果胶杆菌（Pectobacterium carotovorum subsp. carotovorum）和果胶杆菌（Pectobacterium atrosepticum）的拮抗活性。

Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum.

机构信息

Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Victor Lamas 1290, P.O. Box: 160 C, Concepción, Chile.

Instituto de Investigaciones Agropecuarias, INIA Remehue. Ruta 5 Norte Km 8-, Osorno, Región de Los Lagos, Chile.

出版信息

BMC Microbiol. 2021 Dec 7;21(1):335. doi: 10.1186/s12866-021-02393-x.

DOI:10.1186/s12866-021-02393-x
PMID:34876006
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8650274/
Abstract

BACKGROUND

The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops.

RESULT

The objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation.

CONCLUSIONS

We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg.

摘要

背景

智利（奇洛埃）种植的土生马铃薯（Solanum tuberosum subsp. tuberosum L.）是一种新的、尚未开发的内生菌资源，可以寻找潜在的生物防治剂，以预防马铃薯作物的细菌性疾病，如黑胫病和软腐病。

结果

本研究的目的是从土生马铃薯中选择具有拮抗活性的内生放线菌，以对抗果胶杆菌（Pectobacterium carotovorum subsp. carotovorum）和果胶杆菌（Pectobacterium atrosepticum），并确定其在抑制马铃薯块茎组织软化症状方面的潜力。通过使用粘质沙雷氏菌 ATCC 12472 野生型（WT）生物测定法进行群体感应淬灭活性测定，以及通过针对荧光原位杂交（DOPE-FISH）的寡核苷酸探针双重标记技术对马铃薯根系（S. tuberosum）的定殖行为来确定这种潜力。结果表明，虽然链霉菌 sp. TP199 和链霉菌 sp. A2R31 能够抑制病原体的生长，但只有链霉菌 sp. TP199 分离株能够抑制果胶杆菌的生长并减少块茎组织软化（p≤0.05）。链霉菌 sp. TP199 在体外具有金属依赖性酰基高丝氨酸内酯（AHL）群体感应淬灭活性，并能在接种后 10 天内定植根内皮层。

结论

我们得出结论，智利南部的土生马铃薯含有内生放线菌，是软腐病和黑胫病防治的潜在药剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/3fee431a284c/12866_2021_2393_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/d4a516bd55fe/12866_2021_2393_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/c77548e7a72e/12866_2021_2393_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/b10b67a83a1d/12866_2021_2393_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/f74d950b2702/12866_2021_2393_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/cbd176c21035/12866_2021_2393_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/db5af5045c2e/12866_2021_2393_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/3fee431a284c/12866_2021_2393_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/d4a516bd55fe/12866_2021_2393_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/c77548e7a72e/12866_2021_2393_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/b10b67a83a1d/12866_2021_2393_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/f74d950b2702/12866_2021_2393_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/cbd176c21035/12866_2021_2393_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/db5af5045c2e/12866_2021_2393_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f360/8650274/3fee431a284c/12866_2021_2393_Fig7_HTML.jpg

相似文献

1
Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum.土著马铃薯（Solanum tuberosum subsp. tuberosum L.）内生放线菌对果胶杆菌（Pectobacterium carotovorum subsp. carotovorum）和果胶杆菌（Pectobacterium atrosepticum）的拮抗活性。
BMC Microbiol. 2021 Dec 7;21(1):335. doi: 10.1186/s12866-021-02393-x.
2
Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates.摩洛哥放线菌分离物对胡萝卜软腐果胶杆菌和胡萝卜软腐欧文氏菌引起的马铃薯软腐病的防治。
World J Microbiol Biotechnol. 2012 Jan;28(1):303-11. doi: 10.1007/s11274-011-0820-5. Epub 2011 Jun 23.
3
Bacteriophages Isolated in China for the Control of Pectobacterium carotovorum Causing Potato Soft Rot in Kenya.中国分离的噬菌体用于控制肯尼亚引起马铃薯软腐病的果胶杆菌。
Virol Sin. 2019 Jun;34(3):287-294. doi: 10.1007/s12250-019-00091-7. Epub 2019 Mar 13.
4
A quorum sensing-defective mutant of Pectobacterium carotovorum ssp. brasiliense 1692 is attenuated in virulence and unable to occlude xylem tissue of susceptible potato plant stems.胡萝卜软腐果胶杆菌巴西亚种1692的群体感应缺陷型突变体毒力减弱，无法堵塞易感马铃薯植株茎部的木质部组织。
Mol Plant Pathol. 2017 Jan;18(1):32-44. doi: 10.1111/mpp.12372. Epub 2016 Jun 10.
5
Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds.解淀粉芽胞杆菌 Ar10 产生糖脂类似物对胡萝卜软腐果胶杆菌的生物防治。
Microbiol Res. 2018 Dec;217:23-33. doi: 10.1016/j.micres.2018.08.013. Epub 2018 Aug 31.
6
N,N'-alkylated Imidazolium-derivatives act as quorum-sensing inhibitors targeting the Pectobacterium atrosepticum-induced symptoms on potato tubers.N,N'- 烷基化咪唑鎓衍生物作为群体感应抑制剂，针对马铃薯块茎上的果胶杆菌诱导的症状。
Int J Mol Sci. 2013 Oct 8;14(10):19976-86. doi: 10.3390/ijms141019976.
7
Potential Control of Potato Soft Rot Disease by the Obligate Predators and Like Organisms.专性捕食者和类似生物对马铃薯软腐病的潜在控制。
Appl Environ Microbiol. 2020 Mar 2;86(6). doi: 10.1128/AEM.02543-19.
8
Pectobacterium spp. associated with bacterial stem rot syndrome of potato in Canada.加拿大与马铃薯细菌性茎腐综合征相关的果胶杆菌属。
Phytopathology. 2012 Oct;102(10):937-47. doi: 10.1094/PHYTO-04-12-0083-R.
9
Detection and characterization of bacteria from the potato rhizosphere degrading N-acyl-homoserine lactone.从马铃薯根际中检测和鉴定降解N-酰基高丝氨酸内酯的细菌。
Can J Microbiol. 2006 Oct;52(10):1006-15. doi: 10.1139/w06-062.
10
Colonization patterns of an mCherry-tagged Pectobacterium carotovorum subsp. brasiliense strain in potato plants.mCherry 标记的胡萝卜软腐果胶杆菌亚种菌株在马铃薯植株中的定殖模式。
Phytopathology. 2013 Dec;103(12):1268-79. doi: 10.1094/PHYTO-02-13-0049-R.

引用本文的文献

1
Exploring the potential of endophyte-plant interactions for improving crop sustainable yields in a changing climate.探索内生植物与植物相互作用在气候变化条件下提高作物可持续产量的潜力。
Front Plant Sci. 2024 Mar 20;15:1349401. doi: 10.3389/fpls.2024.1349401. eCollection 2024.
2
Potato Microbiome: Relationship with Environmental Factors and Approaches for Microbiome Modulation.马铃薯微生物组：与环境因素的关系及微生物组调节方法。
Int J Mol Sci. 2024 Jan 6;25(2):750. doi: 10.3390/ijms25020750.
3
Actinobacteria as Effective Biocontrol Agents against Plant Pathogens, an Overview on Their Role in Eliciting Plant Defense.

本文引用的文献

1
Exploring the potentialities of beneficial endophytes for improved plant growth.探索有益内生菌促进植物生长的潜力。
Saudi J Biol Sci. 2020 Dec;27(12):3622-3633. doi: 10.1016/j.sjbs.2020.08.002. Epub 2020 Aug 6.
2
Plants endophytes: unveiling hidden agenda for bioprospecting toward sustainable agriculture.植物内生菌：揭示生物勘探可持续农业的隐藏议程。
Crit Rev Biotechnol. 2020 Dec;40(8):1210-1231. doi: 10.1080/07388551.2020.1808584. Epub 2020 Aug 30.
3
Evaluation of Antagonistic and Plant Growth Promoting Potential of Streptomyces sp. TT3 Isolated from Tea (Camellia sinensis) Rhizosphere Soil.
放线菌作为对抗植物病原体的有效生物防治剂——关于其在引发植物防御中作用的综述
Microorganisms. 2022 Aug 29;10(9):1739. doi: 10.3390/microorganisms10091739.
从茶（Camellia sinensis）根际土壤中分离得到的链霉菌（Streptomyces sp. TT3）的拮抗和促生潜力评价。
Curr Microbiol. 2020 Aug;77(8):1829-1838. doi: 10.1007/s00284-020-02002-6. Epub 2020 Apr 30.
4
Isolation, plant growth-promoting traits, antagonistic effects on clinical and plant pathogenic organisms and identification of actinomycetes from olive rhizosphere.从橄榄根际土壤中分离具有植物促生特性、对临床和植物病原菌具有拮抗作用的放线菌，并对其进行鉴定。
Microb Pathog. 2020 Jun;143:104134. doi: 10.1016/j.micpath.2020.104134. Epub 2020 Mar 10.
5
Isolation and Characterization of Root-Associated Bacterial Endophytes and Their Biocontrol Potential against Major Fungal Phytopathogens of Rice ( L.).水稻根际细菌内生菌的分离、鉴定及其对水稻主要真菌病原菌的生物防治潜力
Pathogens. 2020 Feb 28;9(3):172. doi: 10.3390/pathogens9030172.
6
A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulata.一种用于生物防治由胶孢炭疽菌引起的草莓炭疽病的新型内生链霉菌菌株。
Microbiol Res. 2020 Feb 7;234:126428. doi: 10.1016/j.micres.2020.126428.
7
Revisiting a Key Innovation in Evolutionary Biology: Felsenstein's "Phylogenies and the Comparative Method".重温进化生物学的一个关键创新：费雪斯坦的“系统发育与比较方法”。
Am Nat. 2019 Jun;193(6):755-772. doi: 10.1086/703055. Epub 2019 Apr 23.
8
What is the proper way to apply the multiple comparison test?应用多重比较检验的正确方法是什么？
Korean J Anesthesiol. 2018 Oct;71(5):353-360. doi: 10.4097/kja.d.18.00242. Epub 2018 Aug 28.
9
Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches.植物内生放线菌在特殊及其他生态位中的多样性与应用
Front Microbiol. 2018 Aug 8;9:1767. doi: 10.3389/fmicb.2018.01767. eCollection 2018.
10
Exploitation of endophytes for sustainable agricultural intensification.利用内生菌实现可持续农业集约化。
Mol Plant Pathol. 2017 Apr;18(3):469-473. doi: 10.1111/mpp.12483. Epub 2016 Dec 4.