Real-Time Analyzers, Inc., Middletown, CT 06457, USA.
Pharmaceutics. 2011 Jul 13;3(3):425-39. doi: 10.3390/pharmaceutics3030425.
The number of drug-related emergency room visits in the United States doubled from 2004 to 2009 to 4.6 million. Consequently there is a critical need to rapidly identify the offending drug(s), so that the appropriate medical care can be administered. In an effort to meet this need we have been investigating the ability of surface-enhanced Raman spectroscopy (SERS) to detect and identify numerous drugs in saliva at ng/mL concentrations within 10 minutes. Identification is provided by matching measured spectra to a SERS library comprised of over 150 different drugs, each of which possess a unique spectrum. Trace detection is provided by fused gold colloids trapped within a porous glass matrix that generate SERS. Speed is provided by a syringe-driven sample system that uses a solid-phase extraction capillary combined with a SERS-active capillary in series. Spectral collection is provided by a portable Raman analyzer. Here we describe successful measurement of representative illicit, prescribed, and over-the-counter drugs by SERS, and 50 ng/mL cocaine in saliva as part of a focused study.
在美国，与药物相关的急诊室就诊人数从 2004 年到 2009 年翻了一番，达到 460 万。因此，迫切需要快速识别出导致问题的药物，以便提供适当的医疗护理。为了满足这一需求，我们一直在研究表面增强拉曼光谱（SERS）在 10 分钟内从唾液中以 ng/mL 浓度检测和识别众多药物的能力。通过将测量的光谱与包含 150 多种不同药物的 SERS 库进行匹配来实现识别，每种药物都具有独特的光谱。通过捕获在多孔玻璃基质中的融合金胶体提供痕量检测，这些胶体可产生 SERS。速度由注射器驱动的样品系统提供，该系统使用固相萃取毛细管与串联的 SERS 活性毛细管相结合。光谱采集由便携式拉曼分析仪提供。在这里，我们描述了 SERS 对代表性的非法、处方和非处方药物以及唾液中 50ng/mL 可卡因的成功测量，这是一项重点研究的一部分。
