Station d'Amelioration des Plantes, I.N.R.A., BV 1540, 21034, Dijon-Cedex, France.
Plant Mol Biol. 1985 Jan;4(1):31-7. doi: 10.1007/BF02498713.
Proteins associated with the hypersensitive response (b-proteins) were purified from variousNicotiana species and compared biochemically and serologically. The method developed to purify proteins b1, b2 and b3 ofN. tabacum cv. Xanthi-nc was used to purify b-proteins present inN. sylvestris (b0, b1 and b3) andN. tomentosiformis (b2), the parental species ofN. tabacum, and b1″ from bothN. glutinosa andN. debneyi. Ultracentrifugation and amino acid analysis of some of these proteins has shown that they are very similar and that they are all monomers in their native form (mol wt = 15 700 for b0, b1, b2 and b3; mol wt = 13 800 for b1″).Based on their reactions to an antiserum produced against protein b1 ofN. tabacum cv. Xanthi-nc, 3 serological groups can be recognized which are independent of the source species (I) b0 and b1, (II) b1″ and b2, (III) b3. Thus, proteins in the same serological group but from different species are more closely related than the b-proteins in different serological groups but present in the same species. The implication of this site on the possible phylogeny of b-proteins is discussed.Serological tests confirmed the b-protein present as a constitutive component in the virus resistant interspecific hybrids ofN. glutinosa ×N. debneyi as protein b1″.
与过敏反应相关的蛋白质（b 蛋白）从各种烟草属物种中被分离出来，并在生化和血清学上进行了比较。本研究开发了从烟草品种 Xanthi-nc 中纯化 b1、b2 和 b3 蛋白的方法，并将其应用于纯化野生烟草（b0、b1 和 b3）和烟草属的亲本种毛状烟草（b2）中的 b 蛋白，以及来源于菘蓝和印度菘蓝的 b1″。这些蛋白的超速离心和氨基酸分析表明，它们非常相似，并且在其天然形式下均为单体（b0、b1、b2 和 b3 的分子量为 15700；b1″的分子量为 13800）。基于它们对烟草品种 Xanthi-nc 的 b1 蛋白抗血清的反应，可以识别出 3 个血清学组，它们与来源物种无关（I）b0 和 b1，（II）b1″和 b2，（III）b3。因此，来自不同物种的同一血清学组中的蛋白比来自同一物种的不同血清学组中的 b 蛋白更为密切相关。讨论了该位点对 b 蛋白可能的系统发育的影响。血清学测试证实，在菘蓝×印度菘蓝的抗病毒种间杂种中，作为组成型成分存在的 b 蛋白是 b1″。
