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在被优雅内脐蠕孢菌感染的烟草根中检测PR-b蛋白。

Detection of PR-b proteins in tobacco roots infected with Chalara elegans.

作者信息

Tahiri-Alaoui A, Dumas E, Gianinazzi S

机构信息

Laboratoire de Phytoparasitologie INRA-CNRS, INRA, Dijon, France.

出版信息

Plant Mol Biol. 1990 May;14(5):869-71. doi: 10.1007/BF00016522.

DOI:10.1007/BF00016522
PMID:2102866
Abstract

All previous studies on pathogenesis-related (b) protein (PR-b) induction in tobacco have been carried out on leaves or callus tissue. This paper reports the production of PR-b proteins also in roots of tobacco plants (Nicotiana tabacum cv. Xanthi NC) infected with Chalara elegans. Antiserum against PR-b1 reacted with PR-b1, PR-b2 and PR-b3 and gave the same pattern of reaction as for leaves. Antiserum against PR-b5 revealed the presence of PR-b4, PR-b5 and, very weakly, PR-b6 which have been shown to be beta-1,3 glucanases. Antiserum against PR-b7 reacted with both PR-b7 and PR-b8 which are chitinases.

摘要

此前所有关于烟草中病程相关（b）蛋白（PR-b）诱导的研究都是在叶片或愈伤组织上进行的。本文报道了感染雅致帚梗柱孢菌的烟草植株（烟草品种Xanthi NC）的根部也产生了PR-b蛋白。抗PR-b1血清与PR-b1、PR-b2和PR-b3发生反应，且反应模式与叶片相同。抗PR-b5血清显示存在PR-b4、PR-b5，以及非常微弱的PR-b6，这些蛋白已被证明是β-1,3葡聚糖酶。抗PR-b7血清与PR-b7和PR-b8都发生反应，它们都是几丁质酶。

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本文引用的文献

1
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Plant Mol Biol. 1985 Jan;4(1):31-7. doi: 10.1007/BF02498713.
2
Purification and characterization of 8 of the pathogenesis-related proteins in tobacco leaves reacting hypersensitively to tobacco mosaic virus.烟草叶片感染烟草花叶病毒后过敏反应相关 8 种蛋白的纯化与特性分析。
Plant Mol Biol. 1986 Mar;6(2):69-80. doi: 10.1007/BF00027300.
3
Identification, purification, and characterization of pathogenesis-related proteins from virus-infected Samsun NN tobacco leaves.
菊苣体细胞胚胎发生过程中表达的β-1,3-葡聚糖酶的克隆
Plant Mol Biol. 2000 Jan;42(2):377-86. doi: 10.1023/a:1006344024877.
从感染病毒的 Samsun NN 烟草叶片中鉴定、纯化和分析与发病机理相关的蛋白质。
Plant Mol Biol. 1987 Nov;9(6):593-609. doi: 10.1007/BF00020536.
4
Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases.致病相关蛋白的生物学功能：四种烟草致病相关蛋白为几丁质酶。
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6750-4. doi: 10.1073/pnas.84.19.6750.
5
Biological function of ;pathogenesis-related' proteins: four PR proteins of tobacco have 1,3-beta-glucanase activity."pathogenesis-related" 蛋白的生物学功能：烟草中的 4 种 PR 蛋白具有 1,3-β-葡聚糖酶活性。
EMBO J. 1987 Nov;6(11):3209-12. doi: 10.1002/j.1460-2075.1987.tb02637.x.
6
cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products.烟草花叶病毒（TMV）感染烟草诱导产生的六种mRNA的cDNA克隆及其翻译产物的特性分析
EMBO J. 1986 Sep;5(9):2057-61. doi: 10.1002/j.1460-2075.1986.tb04466.x.
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