ELISA 检测烟草系统和局部病毒感染中的 PR（b）蛋白和 TMV。
The detection of PR (b) protein and TMV by ELISA in systemic and localised virus infections of tobacco.
机构信息
Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., U.K..
出版信息
Plant Mol Biol. 1985 Jan;4(1):55-60. doi: 10.1007/BF02498715.
An antiserum was prepared to the b1 protein purified from TMV infectedN. tabacum cv. Xanthi-nc leaves and used to study PR proteins. The Xanthi-nc proteins b2 and b3 were shown to be serologically closely related to b1. Antisera to b1 protein and TMV were used in a F(ab')2 enzyme linked immunosorbent assay to monitor PR protein and TMV concentrations, respectively, during the first 6 days of a systemic TMV infection (cv. Xanthi) and a localised TMV infection (cv. Xanthi-nc).
制备了抗烟草花叶病毒（TMV）感染的黄花烟草 cv. Xanthi-nc 叶片中 b1 蛋白的抗血清，并用于研究 PR 蛋白。结果表明，Xanthi-nc 蛋白 b2 和 b3 与 b1 在血清学上密切相关。在系统感染 TMV（cv. Xanthi）和局部感染 TMV（cv. Xanthi-nc）的前 6 天中，分别使用针对 b1 蛋白和 TMV 的抗血清，在 F(ab')2 酶联免疫吸附测定中监测 PR 蛋白和 TMV 的浓度。
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