Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., U.K..
Plant Mol Biol. 1985 Jan;4(1):55-60. doi: 10.1007/BF02498715.
An antiserum was prepared to the b1 protein purified from TMV infectedN. tabacum cv. Xanthi-nc leaves and used to study PR proteins. The Xanthi-nc proteins b2 and b3 were shown to be serologically closely related to b1. Antisera to b1 protein and TMV were used in a F(ab')2 enzyme linked immunosorbent assay to monitor PR protein and TMV concentrations, respectively, during the first 6 days of a systemic TMV infection (cv. Xanthi) and a localised TMV infection (cv. Xanthi-nc).
制备了抗烟草花叶病毒（TMV）感染的黄花烟草 cv. Xanthi-nc 叶片中 b1 蛋白的抗血清，并用于研究 PR 蛋白。结果表明，Xanthi-nc 蛋白 b2 和 b3 与 b1 在血清学上密切相关。在系统感染 TMV（cv. Xanthi）和局部感染 TMV（cv. Xanthi-nc）的前 6 天中，分别使用针对 b1 蛋白和 TMV 的抗血清，在 F(ab')2 酶联免疫吸附测定中监测 PR 蛋白和 TMV 的浓度。
