Institut de Biologie Moléculaire et Cellulaire du CNRS, Université Louis Pasteur, 15 rue Descartes, 67084, Strasbourg, France.
Plant Mol Biol. 1985 Sep;4(5):305-10. doi: 10.1007/BF02418249.
The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one of the inverted repeats and the gene for the α subunit of ATP synthase. They are located on two Bam H1 fragments, called B6 and B18 by Bowmanet al. (1), which are separated by about 450 bp and which were cloned in our laboratory to allow sequencing. ThetrnD-GUC andtrnC-GCA sequences show 98.6 and 89% homology, respectively, with the corresponding spinach chloroplast tRNA genes sequences (2), which are the only other higher plant chloroplasttrnD-GUC andtrnC-GCA sequenced so far, while no othertrnG-GCC sequence has been published. ThetrnG-GCC sequence shows only 58% homology with the corresponding gene sequence inEuglena chloroplasts (3).
已确定编码 tRNA GCC（Gly）（trnG-GCC）、tRNA GUC（Asp）（trnD-GUC）和 tRNA GCA（Cys）（trnC-GCA）的基因在小麦叶绿体 DNA 图谱上的位置和核苷酸序列。这三个基因位于叶绿体基因组的大单拷贝区，大约在两个反向重复之间的一个和 ATP 合酶α亚基基因之间的一半位置。它们位于 Bowman 等人（1）称为 B6 和 B18 的两个 Bam H1 片段上（1），这两个片段之间相隔约 450bp，我们实验室克隆了这两个片段以允许测序。trnD-GUC 和 trnC-GCA 序列分别与菠菜叶绿体 tRNA 基因序列（2）具有 98.6%和 89%的同源性，这是迄今为止仅有的其他高等植物叶绿体 trnD-GUC 和 trnC-GCA 测序序列，而尚未公布其他 trnG-GCC 序列。trnG-GCC 序列与 Euglena 叶绿体中相应的基因序列（3）仅具有 58%的同源性。
