Izuchi S, Terachi T, Sakamoto M, Mikami T, Sugita M
Department of Botany, Faculty of Science, Hokkaido University, Sapporo, Japan.
Curr Genet. 1990 Oct;18(3):239-43. doi: 10.1007/BF00318387.
The nucleotide sequences of tRNA(Asn) (GUU) and tRNA(Tyr) (GUA) genes from tomato mitochondria and their flanking regions have been determined. The tomato mitochondrial tRNA(Asn) gene is located 2.1 kb downstream from the tRNA(Cys) gene reported previously (Izuchi and Sugita 1989) and shows a nearly complete identity with the corresponding chloroplast gene. The tRNA(Tyr) gene, which shows only 73% homology with the corresponding chloroplast gene, has to be considered a "native" mitochondrial tRNA gene and is 535 bp from the "chloroplast-like" tRNA(Asn) gene on the same strand. Northern hybridization analysis revealed that the three tRNA genes are transcribed in tomato mitochondria. Southern hybridization analysis of tomato, sugar beet. rice and wheat mitochondrial DNAs, with oligonucleotide probes for mitochondrial or chloroplast tRNA genes, demonstrated that the mitochondrial tRNA(Cys) gene found in tomato is present in dicot plants but not in monocots. On the other hand, a chloroplast-like tRNA(Cys) gene exists in monocot plants.
已确定番茄线粒体中tRNA(Asn)(GUU)和tRNA(Tyr)(GUA)基因及其侧翼区域的核苷酸序列。番茄线粒体tRNA(Asn)基因位于先前报道的tRNA(Cys)基因下游2.1 kb处（Izuchi和Sugita，1989），与相应的叶绿体基因几乎完全相同。tRNA(Tyr)基因与相应的叶绿体基因仅有73%的同源性，必须被视为“天然”线粒体tRNA基因，并且在同一条链上距离“叶绿体样”tRNA(Asn)基因535 bp。Northern杂交分析表明，这三个tRNA基因在番茄线粒体中被转录。用针对线粒体或叶绿体tRNA基因的寡核苷酸探针，对番茄、甜菜、水稻和小麦线粒体DNA进行Southern杂交分析，结果表明，番茄中发现的线粒体tRNA(Cys)基因存在于双子叶植物中，而不存在于单子叶植物中。另一方面，单子叶植物中存在叶绿体样tRNA(Cys)基因。
