Instituto di Genetica della Università, Via Matteotti 1/A, I-56100, Pisa, Italy.
Planta. 1979 Oct;147(1):27-9. doi: 10.1007/BF00384586.
Phaseolus coccineus embryos at the heartshaped and the middle cotyledonary stages were cultured in vitro on media added with different concentrations of zeatin (Z) or zeatin riboside (Zr). Growth of early embryos was clearly favored by concentrations of Z from 10(-8) M to 10(-5) M, lower concentrations having no effect. Zr also promoted in vitro growth of early embryos, but in concentrations from 10(-12) M to 10(-10) M, higher concentrations being inhibitory. More developed embryos were scarcely sensitive to the presence in the culture medium of either Z or Zr at any concentration.
红花菜豆心形期和子叶中期胚在添加不同浓度玉米素(Z)或玉米素核苷(Zr)的培养基上进行体外培养。浓度为 10(-8) M 至 10(-5) M 的 Z 明显促进早期胚胎的生长,较低浓度则没有效果。Zr 也促进早期胚胎的体外生长,但在 10(-12) M 至 10(-10) M 的浓度下,较高浓度则具有抑制作用。在任何浓度下,培养基中存在 Z 或 Zr 对发育程度更高的胚胎几乎没有影响。