Department of Plant Sciences, The University, LS2 9JT, Leeds, U.K..
Planta. 1979 Dec;147(2):174-7. doi: 10.1007/BF00389521.
Protoplasts have been obtained from vegetative thallus of the green seaweed Enteromorpha following enzymic digestion with driselase and pectinase. The viability of purified protoplast fractions was assessed by staining and measurements of O2 uptake and evolution.
已通过用 driselase 和果胶酶进行酶解,从绿色海藻石莼的营养体中获得原生质体。通过染色和测量 O2 摄取和释放来评估纯化原生质体级分的活力。