Taylor-Papadimitriou J, Millis R, Burchell J, Nash R, Pang L, Gilbert J
J Exp Pathol. 1986 Summer;2(4):247-60.
The monoclonal antibodies HMFG-1 and HMFG-2 are directed against different epitopes on a large molecule in the human milk fat globule and are also expressed on breast tumors. Here we examine the relative intensity of staining of the two antibodies in formalin fixed paraffin embedded tissue sections from malignant and benign mammary lesions. Most ductal carcinomas and their lymph node metastases stained more strongly with HMFG-2 whereas primary lobular carcinomas are more variable but the majority of their lymph node metastases stained more strongly with HMFG-1. Western blots of gel separated extracts of formalin fixed, paraffin embedded tumor tissue, stained with the antibodies in an ELISA assay, show that the molecular weight of components expressing the antigenic determinants varies. Smaller molecular weight components recognised by HMFG-2 and present in metastatic tumors may be lost during tissue processing. To assess the importance of the molecular weight parameter in tumor classification and prognosis it will be necessary to use frozen tissue.
单克隆抗体HMFG - 1和HMFG - 2针对人乳脂肪球中一种大分子上的不同表位，且在乳腺肿瘤中也有表达。在此，我们检测了这两种抗体在来自恶性和良性乳腺病变的福尔马林固定石蜡包埋组织切片中的相对染色强度。大多数导管癌及其淋巴结转移灶用HMFG - 2染色更强，而原发性小叶癌的情况则更具变异性，但它们的大多数淋巴结转移灶用HMFG - 1染色更强。用抗体在ELISA分析中对福尔马林固定、石蜡包埋肿瘤组织的凝胶分离提取物进行蛋白质免疫印迹，结果显示表达抗原决定簇的成分分子量有所不同。HMFG - 2识别的、存在于转移瘤中的较小分子量成分可能在组织处理过程中丢失。为评估分子量参数在肿瘤分类和预后中的重要性，有必要使用冷冻组织。
