采用酶联免疫吸附测定法（ELISA）和高效液相色谱法（HPLC）测定大不里士糖果坚果中的黄曲霉毒素。
Determination of aflatoxins in nuts of Tabriz confectionaries by ELISA and HPLC methods.
作者信息
Siahi Shadbad Mohammad Reza, Ansarin Masoud, Tahavori Ali, Ghaderi Faranak, Nemati Mahboob
机构信息
Department of Pharmaceutical and Food Control, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Adv Pharm Bull. 2012;2(1):123-6. doi: 10.5681/apb.2012.018. Epub 2012 May 10.
PURPOSE
Aflatoxins (AFs) are a group of mycotoxins and secondary metabolites of various species of Aspergillus. There are various forms of aflatoxins including B1, B2, G1, G2, M1 and M2 types. Aflatoxins cause important health problems and have high potential effect on liver cancer. Therefore, numerous investigations have been conducted during last three decades. The aim of this work is to determine the contamination levels of nuts used by the confectionaries in Tabriz.
METHOD
A total of 142 samples including 35 almond , 26 walnut, 4 seeds of apricot, 6 sunflower seeds kernel, 6 sesame seed, 6 peanuts , 32 pistachio,13 hazelnuts and 14 cashews samples were collected from Tabriz confectionaries. The ELISA method was employed for the screening of total aflatoxins.
RESULT
In 13 cases (28.1% of pistachios, 5.1% of walnuts and 7.1% of cashews) contamination rate of higher than 15 ppb were observed. The HPLC method was applied for the confirmation of ELISA results. Aflatoxin B1 was the highest detected AFs.
CONCLUSION
The overall results of the tested samples indicated that the rate of contamination of pistachios is higher than the other tested samples.
目的
黄曲霉毒素（AFs）是一组霉菌毒素，是曲霉菌各种菌株的次生代谢产物。黄曲霉毒素有多种形式，包括B1、B2、G1、G2、M1和M2型。黄曲霉毒素会引发重要的健康问题，对肝癌具有很高的潜在影响。因此，在过去三十年中进行了大量调查。这项工作的目的是确定大不里士糖果店所使用坚果的污染水平。
方法
从大不里士的糖果店收集了总共142个样本，包括35个杏仁、26个核桃、4个杏仁种子、6个葵花籽仁、6个芝麻、6个花生、32个开心果、13个榛子和14个腰果样本。采用酶联免疫吸附测定（ELISA）法筛查总黄曲霉毒素。
结果
在13个样本中（开心果的28.1%、核桃的5.1%和腰果的7.1%）观察到污染率高于15 ppb。采用高效液相色谱（HPLC）法对ELISA结果进行确认。检测到的黄曲霉毒素中黄曲霉毒素B1含量最高。
结论
测试样本的总体结果表明，开心果的污染率高于其他测试样本。
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