a Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic.
Can J Microbiol. 2013 Dec;59(12):797-802. doi: 10.1139/cjm-2013-0635. Epub 2013 Oct 24.
This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA-PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells.
本文研究了弯曲杆菌属细菌生物膜的形成。引入并优化了一种多重聚合酶链反应（PCR）方法，用于检测生物膜，同时使用嵌入染料吖啶橙（EMA）和吖啶丙二酰胺（PMA），首先对来自收集的弯曲杆菌属菌株进行分析，然后应用于制备生物膜的样品。研究结果表明，弯曲杆菌属内的细菌物种存在相当大的变异性。EMA-PMA PCR 方法可以区分活细胞和死细胞，因此适用于确定细胞的活力。
