健康绝经前妇女上生殖道中 CD4+ 和 CD8+ T 细胞的表型和功能。
Phenotype and functionality of CD4+ and CD8+ T cells in the upper reproductive tract of healthy premenopausal women.
机构信息
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA.
出版信息
Am J Reprod Immunol. 2014 Feb;71(2):95-108. doi: 10.1111/aji.12182. Epub 2013 Dec 6.
PROBLEM
The goal of this study was to investigate the phenotype and functional responsiveness of CD4(+) and CD8(+) T-cells in the upper reproductive tract of healthy premenopausal women. The lower reproductive tract is frequently studied as a site of sexually transmitted infections; however, the upper reproductive tract may also be a portal of entry and dissemination for pathogens, including HIV-1.
METHOD OF STUDY
Endometrial biopsy, endocervical curettage, cytobrush, and blood were collected during mid-luteal phase from 23 healthy women. T-cells were isolated and analyzed by flow cytometry.
RESULTS
As compared with their counterparts in blood, endometrial and endocervical T-cells had enhanced CCR5 expression, and were enriched for activated, effector memory cells. Endometrial T-cells were more responsive to polyclonal stimuli, producing a broad range of cytokines and chemokines.
CONCLUSION
These findings underscore the responsiveness of endometrial T-cells to stimulation, and reveal their activated phenotype. These findings also suggest susceptibility of the upper reproductive tract to HIV-1 infection.
问题
本研究旨在研究健康绝经前妇女上生殖道中 CD4(+)和 CD8(+)T 细胞的表型和功能反应性。下生殖道通常被作为性传播感染的研究部位；然而，上生殖道也可能是病原体（包括 HIV-1）进入和传播的门户。
研究方法
从 23 名健康女性的黄体中期采集子宫内膜活检、宫颈刮宫、宫颈刷和血液。通过流式细胞术分离和分析 T 细胞。
结果
与血液中的对应细胞相比，子宫内膜和宫颈 T 细胞表达增强了 CCR5，并且富含活化的、效应记忆细胞。子宫内膜 T 细胞对多克隆刺激的反应更敏感，产生广泛的细胞因子和趋化因子。
结论
这些发现强调了子宫内膜 T 细胞对刺激的反应性，并揭示了它们的活化表型。这些发现还表明上生殖道易感染 HIV-1。
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