人类女性生殖道中的病原体识别：细胞内细胞质传感器 NOD1、NOD2、RIG-1 和 MDA5 的表达及对 HIV-1 和淋病奈瑟菌的反应。

Pathogen recognition in the human female reproductive tract: expression of intracellular cytosolic sensors NOD1, NOD2, RIG-1, and MDA5 and response to HIV-1 and Neisseria gonorrhea.

机构信息

Department of Epidemiology and Biostatistics, The George Washington University, Washington, DC 20037, USA.

出版信息

Am J Reprod Immunol. 2013 Jan;69(1):41-51. doi: 10.1111/aji.12019. Epub 2012 Sep 17.

DOI:10.1111/aji.12019

PMID:22984986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3518676/

Abstract

PROBLEM

Expression patterns and regulation of cytosolic pattern recognition receptors (PRR) NOD-1, NOD-2, RIG-1, and MDA5 have not been elucidated in the human female reproductive tract (FRT).

METHOD OF STUDY

Primary epithelial cells (EC) isolated from Fallopian tube (FT), endometrium (EM), cervix (Cx), and ectocervix (Ecx) were treated with estradiol, poly(I:C), Neisseria gonorrhea (GC), and HIV-1. PRR mRNA expressions were analyzed by Real-time RT-PCR. Conditioned media were analyzed for IL-8 by ELISA.

RESULTS

EC from all FRT compartments constitutively expressed NOD1, NOD2, RIG-1, and MDA5 with highest levels expressed by FT. Stimulation with poly(I:C) resulted in upregulation of NOD2, RIG-1, and MDA5 in all FRT compartments and correlated with increased secretion of IL-8, whereas estradiol treatment had no effects. Exposure to GC and HIV-1 IIIB but not BaL resulted in selective upregulation of NOD2 and MDA5.

CONCLUSION

PRR are expressed throughout the FRT and differentially regulated by poly(I:C), GC and HIV-1.

摘要

问题

细胞质模式识别受体（PRR）NOD-1、NOD-2、RIG-1 和 MDA5 的表达模式和调控在女性生殖道（FRT）中尚未阐明。

方法

从输卵管（FT）、子宫内膜（EM）、宫颈（Cx）和阴道外口（Ecx）分离的原代上皮细胞（EC）用雌二醇、poly（I：C）、淋病奈瑟菌（GC）和 HIV-1 处理。通过实时 RT-PCR 分析 PRR mRNA 的表达。通过 ELISA 分析条件培养基中的 IL-8。

结果

所有 FRT 区室的 EC 均持续表达 NOD1、NOD2、RIG-1 和 MDA5，FT 表达水平最高。poly（I：C）刺激导致所有 FRT 区室的 NOD2、RIG-1 和 MDA5 上调，并与 IL-8 的分泌增加相关，而雌二醇处理没有影响。GC 和 HIV-1 IIIB 但不是 BaL 的暴露导致 NOD2 和 MDA5 的选择性上调。

结论

PRR 在整个 FRT 中表达，并通过 poly（I：C）、GC 和 HIV-1 进行差异调节。

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人类女性生殖道中的病原体识别：细胞内细胞质传感器 NOD1、NOD2、RIG-1 和 MDA5 的表达及对 HIV-1 和淋病奈瑟菌的反应。

Pathogen recognition in the human female reproductive tract: expression of intracellular cytosolic sensors NOD1, NOD2, RIG-1, and MDA5 and response to HIV-1 and Neisseria gonorrhea.

机构信息

Department of Epidemiology and Biostatistics, The George Washington University, Washington, DC 20037, USA.

出版信息

Am J Reprod Immunol. 2013 Jan;69(1):41-51. doi: 10.1111/aji.12019. Epub 2012 Sep 17.

DOI:10.1111/aji.12019

PMID:22984986

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3518676/

Abstract

PROBLEM

Expression patterns and regulation of cytosolic pattern recognition receptors (PRR) NOD-1, NOD-2, RIG-1, and MDA5 have not been elucidated in the human female reproductive tract (FRT).

METHOD OF STUDY

RESULTS

CONCLUSION

PRR are expressed throughout the FRT and differentially regulated by poly(I:C), GC and HIV-1.

摘要

问题

细胞质模式识别受体（PRR）NOD-1、NOD-2、RIG-1 和 MDA5 的表达模式和调控在女性生殖道（FRT）中尚未阐明。

方法

结果

结论

PRR 在整个 FRT 中表达，并通过 poly（I：C）、GC 和 HIV-1 进行差异调节。

人类女性生殖道中的病原体识别：细胞内细胞质传感器 NOD1、NOD2、RIG-1 和 MDA5 的表达及对 HIV-1 和淋病奈瑟菌的反应。

Pathogen recognition in the human female reproductive tract: expression of intracellular cytosolic sensors NOD1, NOD2, RIG-1, and MDA5 and response to HIV-1 and Neisseria gonorrhea.

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出版信息

PROBLEM

METHOD OF STUDY

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人类女性生殖道中的病原体识别：细胞内细胞质传感器 NOD1、NOD2、RIG-1 和 MDA5 的表达及对 HIV-1 和淋病奈瑟菌的反应。

Pathogen recognition in the human female reproductive tract: expression of intracellular cytosolic sensors NOD1, NOD2, RIG-1, and MDA5 and response to HIV-1 and Neisseria gonorrhea.

机构信息

出版信息

PROBLEM

METHOD OF STUDY

RESULTS

CONCLUSION

问题

方法

结果

结论