Tamaddonfard Esmaeal, Farshid Amir Abbas, Maroufi Shirin, Kazemi-Shojaei Sharare, Erfanparast Amir, Asri-Rezaei Siamak, Taati Mina, Dabbaghi Milad, Escort Mona
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran.
Division of Pathology, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia 57153-1177, Iran.
Phytomedicine. 2014 Apr 15;21(5):717-23. doi: 10.1016/j.phymed.2013.10.031. Epub 2013 Dec 4.
Safranal is one of the major components of saffron and has many biological effects such as antioxidant property. The present study investigated the effects of safranal on sciatic nerve function after induction of crush injury. We also used of vitamin E as a reference potent antioxidant agent. In anesthetized rats, right sciatic nerve was crushed using a small haemostatic forceps. Functional recovery was assessed using sciatic functional index (SFI). Acetone spray and von Frey filament tests were used for neuropathic pain assay. Histopathological changes including severities of Wallerian degeneration of sciatic nerve and gastrocnemius muscle atrophy were investigated by light microscopy. Blood levels of malodialdehyde (MDA) were also measured. The SFI values were accelerated, cold and mechanical allodynia were suppressed, the severities of Wallerian degeneration and muscular atrophy were improved, and the increased MDA level was reversed with 10 consecutive days intraperitoneal injections of 0.2 and 0.8 mg/kg of safranal and 100 mg/kg of vitamin E. It is concluded that safranal and vitamin E produced same improving effects on crushed-injured sciatic nerve functions. Inhibition of oxidative stress pathway may be involved in improving effects of safranal and vitamin E on functions and histopathology of an injured peripheral nerve.
藏红花醛是藏红花的主要成分之一，具有许多生物学效应，如抗氧化特性。本研究调查了藏红花醛对挤压伤后坐骨神经功能的影响。我们还使用维生素E作为参考强效抗氧化剂。在麻醉大鼠中，用小型止血钳挤压右侧坐骨神经。使用坐骨神经功能指数（SFI）评估功能恢复情况。使用丙酮喷雾和von Frey细丝试验进行神经性疼痛测定。通过光学显微镜研究组织病理学变化，包括坐骨神经华勒氏变性的严重程度和腓肠肌萎缩。还测量了血液中丙二醛（MDA）的水平。连续10天腹腔注射0.2和0.8mg/kg藏红花醛以及100mg/kg维生素E后，SFI值加快，冷和机械性异常性疼痛受到抑制，华勒氏变性和肌肉萎缩的严重程度得到改善，升高的MDA水平恢复正常。得出的结论是，藏红花醛和维生素E对挤压伤的坐骨神经功能产生相同的改善作用。氧化应激途径的抑制可能参与了藏红花醛和维生素E对受损周围神经功能和组织病理学的改善作用。
