Mohebbati Reza, Kamkar-Del Yasamin, Shafei Mohammad Naser, Rakhshandeh Hasan, Aghaei Azita
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Vet Res Forum. 2021 Spring;12(2):185-190. doi: 10.30466/vrf.2019.103224.2456. Epub 2021 Jun 15.
The saffron petals are a by-product part of the saffron flower with a cardiovascular effect. This study evaluated the effect of the saffron petal on hypertension induced by angiotensin II (AII) and NG-nitro-L-arginine methyl ester (L-NAME, a NOS inhibitor). Rats were divided into 11 groups: 1) Control, 2) AII (50.00 ng kg), 3) Losartan+ AII, 4) L-NAME (10.00 mg kg), 5) sodium nitroprusside (SNP) + L-NAME, 6, 7) Saffron petals extract; 8, 9) saffron petals (100 and 200 mg kg) + AII and 10,11) saffron petals (100 and 200 mg kg) + L-NAME. Hypertension induced by intravenous injection of AII and L-NAME in separate groups. In treated groups, 30 min before injection of AII or L-NAME rats received two doses of extract via intraperitoneal administration. The femoral artery was cannulated and cardiovascular parameters recorded by a transducer connected to power lab apparatus. Maximal changes (∆) of mean arterial pressure (MAP), systolic blood pressure (SBP) and heart rate (HR) from baseline were calculated and compared to with those in hypertensive and control groups. Results showed that both AII and L-NAME significantly increased SBP and MAP than control, however, HR in AII was decreased and in the L-NAME group increased. Pre-treatment with saffron petals could significantly attenuate the cardiovascular responses induced by both AII and L-NAME. However, the effect of the extract in AII hypertensive rats was more effective than L-NAME groups. The findings showed that the hydroalcoholic extract of the saffron petals had an antihypertensive effect that mainly was mediated by inhibition of AII activity.
藏红花花瓣是藏红花花朵的一种具有心血管效应的副产品。本研究评估了藏红花花瓣对血管紧张素II（AII）和NG-硝基-L-精氨酸甲酯（L-NAME，一种一氧化氮合酶抑制剂）诱导的高血压的影响。大鼠被分为11组：1）对照组，2）AII（50.00 ng/kg）组，3）氯沙坦+AII组，4）L-NAME（10.00 mg/kg）组，5）硝普钠（SNP）+L-NAME组，6、7）藏红花花瓣提取物组；8、9）藏红花花瓣（100和200 mg/kg）+AII组以及10、11）藏红花花瓣（100和200 mg/kg）+L-NAME组。在不同组中通过静脉注射AII和L-NAME诱导高血压。在治疗组中，在注射AII或L-NAME前30分钟，大鼠通过腹腔注射接受两剂提取物。将股动脉插管，并通过连接到PowerLab仪器的传感器记录心血管参数。计算平均动脉压（MAP）、收缩压（SBP）和心率（HR）相对于基线的最大变化（∆），并与高血压组和对照组进行比较。结果显示，与对照组相比，AII和L-NAME均显著升高SBP和MAP，然而，AII组的HR降低，而L-NAME组的HR升高。用藏红花花瓣预处理可显著减弱AII和L-NAME诱导的心血管反应。然而，提取物对AII高血压大鼠的作用比L-NAME组更有效。研究结果表明，藏红花花瓣的水醇提取物具有降压作用，其主要通过抑制AII活性介导。
