Döpel S H, Töpel K, Schulz J, Schneider W, Ladhoff A, Naumann W
Zentralbl Allg Pathol. 1986;132(3):197-207.
Rabbits were immunized with 10 nm filaments of a mixture of cytokeratins which has been isolated from human heel callus material and reconstituted to filaments in vitro. The antisera to keratins (ASK) have been tested histologically at fixed and unfixed tissue samples by means of the indirect immunofluorescence and PAP technique. The ASK recognized specifically only the epithelial cells of skin, of the mucous membranes of mouth and digestive tract, of salivary glands, sweat gland and mammary gland, but did not react with hepatocytes or kidney cells. The following tumors, tested till now, reacted with the antikeratin antisera: epithelial and lymphoepithelial carcinomas of skin, mouth and digestive tract, carcinomas of salivary glands, mammary gland and thyroid gland, adamantinoma, basalioma of skin, and metastases from carcinomas.
用从人足跟胼胝组织中分离出来并在体外重构成细丝的细胞角蛋白混合物的10纳米细丝对兔子进行免疫。通过间接免疫荧光和PAP技术，在固定和未固定的组织样本上对角蛋白抗血清（ASK）进行了组织学检测。ASK仅特异性识别皮肤、口腔和消化道黏膜、唾液腺、汗腺和乳腺的上皮细胞，而不与肝细胞或肾细胞发生反应。到目前为止所检测的下列肿瘤与抗角蛋白抗血清发生反应：皮肤、口腔和消化道的上皮癌和淋巴上皮癌、唾液腺、乳腺和甲状腺癌、造釉细胞瘤、皮肤基底细胞瘤以及癌转移瘤。
