Department of Chemistry and Research Center for Analysis and Identification, Chung Yuan Christian University, 200 Chung Pei Road, Chungli 32023, Taiwan, ROC.
Enzyme Microb Technol. 2013 Dec 10;53(6-7):444-51. doi: 10.1016/j.enzmictec.2013.09.010. Epub 2013 Sep 30.
Cellulase immobilized on silica through the assistance of l-cysteine functionalized gold nano-particle was applied for the continuous hydrolysis of waste bamboo chopsticks powder to produce glucose. The optimal conditions for the continuous hydrolysis were pH 8.0, 50°C. A 4-day reaction with an initial 0.3 gL⁻¹ waste bamboo chopsticks powder, a feed containing 0.2 gL⁻¹ waste bamboo chopsticks powder at a continuous feed and draw rate of 0.5 mLmin⁻¹, and an enzyme loading of 40 mgcellulase(gsilica)⁻¹, has 72.0-76.6% conversion rates of repeated hydrolyses that correspond to a total production of 630.5-671.2mg glucose and are much better than batch hydrolyses. At higher enzyme loading (117 mgcellulase(gsilica)⁻¹), higher initial concentration (0.5 gL⁻¹), and higher feed concentration (0.42 gL⁻¹) the conversion rate increases to 82.9% and a total amount of 1418 mgglucose. The immobilized cellulase can be recovered easily by filtration and used repeatedly at least 6 times over a period more than 90 days with a recovered activity approximately the same as or better than previous reactions. Thus the process is promising for scaling up.
通过 l-半胱氨酸功能化金纳米粒子的辅助，将纤维素酶固定在硅胶上，用于连续水解废竹筷粉生产葡萄糖。连续水解的最佳条件为 pH8.0、50°C。在初始浓度为 0.3 gL⁻¹ 的废竹筷粉、以 0.5 mLmin⁻¹ 的连续进料和出料速率进料浓度为 0.2 gL⁻¹、酶载量为 40 mgcellulase(gsilica)⁻¹ 的条件下，进行 4 天反应，重复水解的转化率为 72.0-76.6%，对应的总葡萄糖产量为 630.5-671.2mg，明显优于分批水解。在较高的酶载量（117 mgcellulase(gsilica)⁻¹）、较高的初始浓度（0.5 gL⁻¹）和较高的进料浓度（0.42 gL⁻¹）下，转化率提高到 82.9%，总葡萄糖量为 1418 mgglucose。固定化纤维素酶可通过过滤轻松回收，在 90 天以上的时间内至少重复使用 6 次，回收活性与之前的反应相近或更好。因此，该工艺具有扩大规模的潜力。
