[家用灰尘制成的商业过敏原制剂在细菌吸附反应中的比活性评估：与放射变应原吸附抑制试验结果的比较]

[Assessment of the specific activity of commercial allergen preparations made of house dust in the bacteriosorption reaction: a comparison with the results of the radioallergosorbent inhibition test].

作者信息

Nikolaev V L, Muzyleva I L, Kapitonova M E, Mats A N

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1986 Sep(9):69-73.

Abstract

The activity of the commercial batches of house-dust (HD) allergens was compared in the inhibition of the radioallergosorbent test (RAST) and in the direct bacteriosorbent test (BST), detecting IgG to the antigens by adsorption on the complexes of whole staphylococcal cells containing protein A. BST was made with rabbit antiserum to HD allergen. This antiserum inhibited RAST by 76% and, therefore, contained antibodies to most of the allergenic determinants of HD. At the same time, no significant correlation between the activity of 15 batches of HD allergen was revealed in RAST inhibition and in BST with the above antiserum. Nevertheless, the exhaustion of the antiserum with a batch of HD allergen showing low activity in RAST inhibition, but high activity in BST made it possible to obtain BST results significantly correlating with the data resulting from RAST inhibition in two series of experiments.

摘要

对市售批次的屋尘（HD）过敏原的活性进行了比较，比较内容包括在放射变应原吸附试验（RAST）中的抑制情况以及在直接细菌吸附试验（BST）中的情况，后者通过吸附在含有蛋白A的全葡萄球菌细胞复合物上来检测针对抗原的IgG。BST是用兔抗HD过敏原血清进行的。该抗血清对RAST的抑制率为76%，因此含有针对HD大多数变应原决定簇的抗体。同时，在RAST抑制试验和使用上述抗血清的BST中，未发现15批次HD过敏原的活性之间存在显著相关性。然而，用一批在RAST抑制试验中活性低但在BST中活性高的HD过敏原使抗血清耗尽后，在两个系列的实验中，能够获得与RAST抑制试验结果显著相关的BST结果。