Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland.
Department of Otorhinolaryngology-Head and Neck Surgery, Helsinki University Central Hospital, Helsinki, Finland.
J Clin Virol. 2014 Jan;59(1):55-8. doi: 10.1016/j.jcv.2013.11.008. Epub 2013 Nov 27.
The trichodysplasia spinulosa-associated polyomavirus (TSPyV), a recently discovered species of the family Polyomaviridae, is associated with development of trichodysplasia spinulosa (TS), a rare follicular skin disease of immunocompromised individuals. The viral seroprevalence in the general population is ∼70%, with little known of its route of transmission, latency, or primary infection site.
We aimed to determine whether the viral DNA is detectable in tonsillar tissue of constitutionally healthy individuals, and what the corresponding antiviral seroreactivities are.
We tested 229 matched pairs of tonsillar tissue biopsies and serum samples from asymptomatic donors for TSPyV DNA by real-time quantitative PCR with primer pairs and Taq-Man probes targeting the VP1 and LT genes. The sera were studied by enzyme immunoassay (EIA) for TSPyV-VP1-IgG and the PCR-positive individuals also for -IgM and -IgG-avidity.
TSPyV DNA was detectable in 8 (3.5%) of 229 tonsillar tissues, and in none of the corresponding sera. TSPyV IgG seroprevalence among children was 39% and among adults 70%. Each of the 8 PCR-positive subjects had antiviral IgG of high avidity but not IgM.
TSPyV PCR positivity of tonsillar samples of individuals with long-term immunity provides the first evidence of TSPyV in tonsils and suggests lymphoid tissue as a latency site of this emerging human pathogen.
新近发现的多瘤病毒科 Trichodysplasia spinulosa 相关多瘤病毒（TSPyV）与 Trichodysplasia spinulosa（TS）的发生有关，TS 是一种罕见的免疫功能低下个体的毛囊皮肤疾病。该病毒在普通人群中的血清阳性率约为 70%，但人们对其传播途径、潜伏期或原发感染部位知之甚少。
我们旨在确定 TSPyV 的 DNA 是否可在健康个体的扁桃体组织中检测到，以及相应的抗病毒血清反应如何。
我们通过针对 VP1 和 LT 基因的实时定量 PCR 用引物对和 Taq-Man 探针检测 229 对来自无症状供体的扁桃体组织活检和血清样本中的 TSPyV DNA。通过酶免疫分析（EIA）检测血清中的 TSPyV-VP1-IgG，PCR 阳性个体还检测 -IgM 和 -IgG-亲和力。
在 229 个扁桃体组织中，有 8 个（3.5%）可检测到 TSPyV DNA，而相应的血清中均不可检测到。儿童 TSPyV IgG 的血清阳性率为 39%，成人则为 70%。8 例 PCR 阳性者均具有高亲和力的抗病毒 IgG，但无 IgM。
长期免疫个体的扁桃体样本中 TSPyV 的 PCR 阳性结果首次证明了 TSPyV 在扁桃体中的存在，并提示淋巴组织可能是这种新兴人类病原体的潜伏部位。
