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金顶侧耳多糖的结构分析及抗氧化活性研究。

Structural analysis and antioxidant activities of polysaccharide isolated from Jinqian mushroom.

机构信息

School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.

School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China.

出版信息

Int J Biol Macromol. 2014 Mar;64:63-8. doi: 10.1016/j.ijbiomac.2013.11.029. Epub 2013 Dec 4.

DOI:10.1016/j.ijbiomac.2013.11.029
PMID:24315944
Abstract

Jinqian mushroom is a precious edible mushroom with delicious taste and high nutritional value. In this paper, a polysaccharide fraction JQPs was isolated and purified from the fruiting body of Jinqian mushroom. The chemical structure, chain conformation and antioxidant activities of JQPs were investigated. The results indicated that JQPs was mainly composed of glucose with trace amounts of xylose. The backbone of JQPs consisted of β-(1 → 3)-D-glucan with β-(1 → 6)-glucosyl side chain. The chain conformation analysis showed that JQPs was a triple helical polysaccharide. The antioxidant activity tests in vitro revealed that JQPs exhibited high DPPH radical and ABTS radical scavenging activities, moderate superoxide radical and hydroxyl radical scavenging activities, low reducing power and Fe(2+) chelating activities. The results suggested that JQPs could be used as a potential natural antioxidant.

摘要

金顶侧耳是一种珍贵的食用蘑菇,味道鲜美,营养价值高。本文从金顶侧耳子实体中分离纯化得到一种多糖组分 JQPs,研究了 JQPs 的化学结构、链构象和抗氧化活性。结果表明,JQPs 主要由葡萄糖组成,含有少量木糖。JQPs 的骨架由β-(1→3)-D-葡聚糖组成,带有β-(1→6)-葡萄糖基侧链。链构象分析表明,JQPs 是一种三螺旋多糖。体外抗氧化活性试验表明,JQPs 具有较高的 DPPH 自由基和 ABTS 自由基清除能力,中等的超氧自由基和羟自由基清除能力,较低的还原能力和 Fe(2+)螯合能力。结果表明,JQPs 可用作一种有潜力的天然抗氧化剂。

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