Bouza Emilio, Martín-Rabadán Pablo, Echenagusia Antonio, Camúñez Fernando, Rodríguez-Rosales Gracia, Simó Gonzalo, Echenagusia Miguel, Guembe María
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, Department of Medicine, Universidad Complutense, Madrid, Spain; Red Española de Investigación en Patología Infecciosa (REIPI), RD06/0008/1025, Madrid, Spain.
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, Department of Medicine, Universidad Complutense, Madrid, Spain; Red Española de Investigación en Patología Infecciosa (REIPI), RD06/0008/1025, Madrid, Spain.
Diagn Microbiol Infect Dis. 2014 Feb;78(2):162-7. doi: 10.1016/j.diagmicrobio.2013.11.004. Epub 2013 Nov 14.
Data on microbiological management of withdrawn venous access ports (VAPs) are scarce. The aim of our study was to assess the validity of Gram stain and culture performed on VAPs to detect colonization and VAP-related bloodstream infection (VAP-RBSI). We prospectively performed cultures of the following: catheter tip (roll-plate and sonication), port content aspirate before and after sonication, port sonication fluid (PSF), and port internal surface biofilm (ISB). The gold standard of VAP colonization was positivity of at least 1 of the cultures mentioned above. We collected 223 VAPs in which no single culture had validity values reliable enough to predict colonization and VAP-RBSI. The best validity values were those obtained when cultures of catheter tip (roll-plate), PSF, and port ISB were combined. Cultures from several areas on the VAP are necessary to ensure suitable assessment of colonization and VAP-RBSI.
关于拔除的静脉输液港（VAP）微生物管理的数据很少。我们研究的目的是评估对VAP进行革兰氏染色和培养以检测定植和VAP相关血流感染（VAP-RBSI）的有效性。我们前瞻性地对以下样本进行了培养：导管尖端（滚板法和超声法）、超声前后的端口内容物抽吸物、端口超声液体（PSF）和端口内表面生物膜（ISB）。VAP定植的金标准是上述至少一种培养物呈阳性。我们收集了223个VAP，其中没有一种培养物具有足够可靠的有效性值来预测定植和VAP-RBSI。最佳有效性值是在将导管尖端（滚板法）、PSF和端口ISB的培养结果结合时获得的。对VAP多个部位进行培养对于确保对定植和VAP-RBSI进行适当评估是必要的。
