Kaznowska E, Depciuch J, Szmuc K, Cebulski J
Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszów, Warzywna 1a, 35-959 Rzeszów, Poland; Department of Human Histology, Chair of Morphological Sciences, Faculty of Medicine, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland.
Institute of Nuclear Physics, Polish Academy of Sciences, PL-31342 Krakow, Poland.
J Pharm Biomed Anal. 2017 Feb 5;134:259-268. doi: 10.1016/j.jpba.2016.11.047. Epub 2016 Nov 30.
Colorectal cancer constitutes 33% of all cancer morbidity, so the research of the new methods for colorectal cancer diagnosis and chemotherapy monitoring is gaining its momentum. Diagnostic instruments are being sought, which enable the detection of single malignant cells based on the analysis of tissue material potentially reusable at further stages of diagnostic management. The most common approach to tissue specimen processing is paraffin-embedding. Yet, paraffin may cause background noise in spectroscopic measurements with the wavenumber ranging between 900cm and 3500cm. However, the study by Depciuch et al. (2016) proved that appropriate specimen processing and paraffin-embedding technique as well as a strict measurement methodology may eliminate paraffin vibrations. As a result, spectroscopic measurements may become a reliable and precise method for the diagnosis and treatment monitoring in patients with colorectal cancer as long as the high standards of specimen processing are maintained. Chemotherapy is the main medical treatment in colorectal cancer. Unfortunately, the absence of tools which enable monitoring its efficacy leads to the partial response or non-response frequently seen in affected patients. Hence, diagnostic instruments are also being sought capable of monitoring treatment efficacy so as to enable early changes of chemotherapy regimen thus increasing the chance of cure. The paper aims at comparing the results of FTIR (Fourier Transform Infrared) spectroscopy in several types of colon tissue: healthy colon, cancerous colon, post-chemotherapy colon and healthy surgical margin of colon cancer sample. The obtained FTIR spectra along with the Principal Component Analysis-Linear Discriminant Analysis (PCA-LDC) as well as bandwidth analysis of the primary amide region revealed some differences between the spectra of healthy tissues as compared to cancerous tissues (pre- or post-chemotherapy). Apart from confirming that FTIR spectroscopy is a good source of information on the composition of analysed samples, this fact supports its application as a tool to facilitate understanding the pathophysiology of various conditions and to monitor efficacy of chemotherapy in cancer patients.
结直肠癌占所有癌症发病率的33%，因此，结直肠癌诊断和化疗监测新方法的研究正日益兴起。人们正在寻找能够基于对在诊断管理的进一步阶段可能可重复使用的组织材料进行分析来检测单个恶性细胞的诊断工具。组织标本处理最常用的方法是石蜡包埋。然而，石蜡可能会在波数范围为900厘米至3500厘米的光谱测量中产生背景噪声。不过，德普丘赫等人（2016年）的研究证明，适当的标本处理和石蜡包埋技术以及严格的测量方法可以消除石蜡振动。因此，只要保持高标准的标本处理，光谱测量可能会成为结直肠癌患者诊断和治疗监测的可靠且精确的方法。化疗是结直肠癌的主要医学治疗方法。不幸的是，缺乏能够监测其疗效的工具导致受影响患者中经常出现部分反应或无反应的情况。因此，人们也在寻找能够监测治疗效果的诊断工具，以便能够尽早改变化疗方案，从而增加治愈的机会。本文旨在比较傅里叶变换红外（FTIR）光谱在几种类型结肠组织中的结果：健康结肠、癌性结肠、化疗后结肠以及结肠癌样本的健康手术切缘。获得的FTIR光谱连同主成分分析 - 线性判别分析（PCA - LDC）以及一级酰胺区域的带宽分析揭示了健康组织光谱与癌性组织（化疗前或化疗后）光谱之间的一些差异。除了证实FTIR光谱是分析样本成分的良好信息来源外，这一事实支持了将其用作有助于理解各种病症的病理生理学以及监测癌症患者化疗疗效的工具。
