Suárez Guillaume, Santschi Christian, Slaveykova Vera I, Martin Olivier J F
1] Nanophotonics and Metrology Laboratory, Swiss Federal Institute of Technology Lausanne (EPFL), EPFL-STI-NAM, Station 11, CH-1015 Lausanne, Switzerland [2].
Sci Rep. 2013 Dec 9;3:3447. doi: 10.1038/srep03447.
Reactive oxygen species play a key role in cell signalling and oxidative stress mechanisms, therefore, sensing their production by living organisms is of fundamental interest. Here we describe a novel biosensing method for extracellular detection of endogenous hydrogen peroxide (H2O2). The method is based on the enhancement of the optical absorption spectrum of the hemoprotein cytochrome c when loaded into a highly scattering random medium. Such a configuration enables, in contrast to existing techniques, non-invasive and dynamic detection of the oxidation of cyt c in the presence of H2O2 with unprecedented sensitivity. Dynamic information on the modification of the cell oxidative status of Chlamydomonas reinhardtii, an aquatic green algae, was obtained under oxidative stress conditions induced by the presence of trace concentrations of Cd(II). Furthermore, the dynamics of H2O2 production was investigated under different lighting conditions confirming the impact of Cd(II) on the photosynthetic activity of those phytoplanktonic cells.
活性氧在细胞信号传导和氧化应激机制中起着关键作用，因此，感知生物体对其的产生具有根本重要性。在此，我们描述了一种用于细胞外检测内源性过氧化氢（H2O2）的新型生物传感方法。该方法基于将血蛋白细胞色素c加载到高度散射的随机介质中时其光吸收光谱的增强。与现有技术相比，这种配置能够以前所未有的灵敏度在存在H2O2的情况下对细胞色素c的氧化进行非侵入性和动态检测。在痕量浓度的Cd(II)存在所诱导的氧化应激条件下，获得了关于莱茵衣藻（一种水生绿藻）细胞氧化状态变化的动态信息。此外，研究了不同光照条件下H2O2的产生动态，证实了Cd(II)对那些浮游植物细胞光合活性的影响。
