Verma Anil Kumar, Goyal Arun, Freire Filipe, Bule Pedro, Venditto Immacolata, Brás Joana L A, Santos Helena, Cardoso Vânia, Bonifácio Cecília, Thompson Andrew, Romão Maria João, Prates José A M, Ferreira Luís M A, Fontes Carlos M G A, Najmudin Shabir
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Dec;69(Pt 12):1440-2. doi: 10.1107/S1744309113025050. Epub 2013 Nov 30.
The modular carbohydrate-active enzyme belonging to glycoside hydrolase family 30 (GH30) from Clostridium thermocellum (CtXynGH30) is a cellulosomal protein which plays an important role in plant cell-wall degradation. The full-length CtXynGH30 contains an N-terminal catalytic module (Xyn30A) followed by a family 6 carbohydrate-binding module (CBM6) and a dockerin at the C-terminus. The recombinant protein has a molecular mass of 45 kDa. Preliminary structural characterization was carried out on Xyn30A crystallized in different conditions. All tested crystals belonged to space group P1 with one molecule in the asymmetric unit. Molecular replacement has been used to solve the Xyn30A structure.
来自嗜热栖热放线菌的属于糖苷水解酶家族30（GH30）的模块化碳水化合物活性酶（CtXynGH30）是一种纤维小体蛋白，在植物细胞壁降解中起重要作用。全长CtXynGH30包含一个N端催化模块（Xyn30A），其后是一个6家族碳水化合物结合模块（CBM6）和一个位于C端的dockerin。重组蛋白的分子量为45 kDa。对在不同条件下结晶的Xyn30A进行了初步结构表征。所有测试晶体都属于空间群P1，不对称单元中有一个分子。已使用分子置换法解析Xyn30A的结构。
