用于爆炸物检测的纳米生物探针介导的DNA适配体

Nanobioprobe mediated DNA aptamers for explosive detection.

作者信息

Shorie Munish, Bhalla Vijayender, Pathania Preeti, Suri C Raman

出版信息

Chem Commun (Camb). 2014 Feb 4;50(9):1080-2. doi: 10.1039/c3cc47562j.

Abstract

Specific nucleic acid aptamers using the microtiter plate based modified SELEX method against explosive trinitrotoluene are reported. Efficient partitioning of dsDNA was carried out using streptavidin labeled gold nanoprobes for the selection of specific aptamers. The selected binders having an affinity of ~10(-7) M were used in the newly developed electrochemical aptasensor, exhibiting a detection limit of around 1 ppb for trinitrotoluene.

摘要

报道了使用基于微孔板的改良SELEX方法筛选针对爆炸物三硝基甲苯的特异性核酸适配体。利用链霉亲和素标记的金纳米探针进行双链DNA的高效分离，以筛选特异性适配体。所筛选出的具有约10^(-7) M亲和力的结合物被用于新开发的电化学适配体传感器，该传感器对三硝基甲苯的检测限约为1 ppb。