Wang Haibin, Shen Li, Li Xinming, Sun Minglei
Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Zhengzhou University.
Biosci Biotechnol Biochem. 2013;77(12):2348-55. doi: 10.1271/bbb.130389. Epub 2013 Dec 7.
1'-Acetoxychavicol acetate (ACA), extracted from rhizomes of tropical ginger, possesses antitumor properties against a wide variety of malignancies. MicroRNAs have been found to act as oncogenes and as tumor suppressor genes in the development of cancer. The purpose of this study was to investigate the miRNA involved in the molecular mechanisms of ACA action on tumor inhibition. It was found that ACA significantly inhibited the growth of human head and neck squamous cell carcinoma cell line HN4 and induced cell apoptosis. Further studies indicated that ACA downregulated the expression of miR-23a in HN4 cells. Transfection with anti-miR-23a inhibited the proliferation of HN4 cells and induced cell apoptosis. In addition, phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was confirmed to be the target of miR-23a. Taken together, our findings suggest that ACA might have anticancer effects against human head and neck cancer through downregulation of miR-23a, which can repress tumor suppressor PTEN.
从热带姜的根茎中提取的乙酸1'-乙酰氧基查维醇（ACA）对多种恶性肿瘤具有抗肿瘤特性。微小RNA在癌症发展过程中既可以作为癌基因，也可以作为肿瘤抑制基因发挥作用。本研究的目的是探究参与ACA抑制肿瘤分子机制的微小RNA。研究发现，ACA显著抑制人头颈部鳞状细胞癌细胞系HN4的生长并诱导细胞凋亡。进一步研究表明，ACA下调了HN4细胞中miR-23a的表达。用抗miR-23a转染可抑制HN4细胞的增殖并诱导细胞凋亡。此外，证实10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）是miR-23a的靶标。综上所述，我们的研究结果表明，ACA可能通过下调miR-23a对人头颈部癌具有抗癌作用，miR-23a可抑制肿瘤抑制因子PTEN。
