Hudcova Kristyna, Trnkova Libuse, Kejnovska Iva, Vorlickova Michaela, Gumulec Jaromir, Kizek Rene, Masarik Michal
Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Eur Biophys J. 2015 Apr;44(3):131-8. doi: 10.1007/s00249-015-1008-y. Epub 2015 Feb 4.
In this study we have chosen a new approach and characterized three miRNAs (miR-23a, miR-34a and miR-320a) related to prostate cancer and head and neck cancer by spectral (circular dichroic and UV-absorption spectra) and electrochemical (voltammetry at graphite and mercury electrodes) methods. The spectral and voltammetric results, reflecting different nucleotide sequences of miRNAs, were complemented by the results of DNAs(U) having the same oligonucleotide sequences as miRNAs. The effect of the substitution of ribose for deoxyribose was shown and structural diversity was confirmed. The stability of RNA and DNA(U) was studied using CD and UV-absorption spectroscopy and melting points were calculated. MiRNA-320a with the highest content of guanine provided the highest melting point. With respect to the rapid progress of miRNA electrochemical sensors, our results will be useful for the research and development of sensitive, portable and time-efficient miRNA sensors, which will be able to diagnose cancer and other diseases.
在本研究中，我们采用了一种新方法，通过光谱法（圆二色光谱和紫外吸收光谱）和电化学方法（石墨电极和汞电极伏安法）对与前列腺癌和头颈癌相关的三种微小RNA（miR-23a、miR-34a和miR-320a）进行了表征。光谱和伏安结果反映了微小RNA不同的核苷酸序列，与微小RNA具有相同寡核苷酸序列的DNA（U）的结果对其进行了补充。展示了核糖取代脱氧核糖的效果并证实了结构多样性。使用圆二色光谱和紫外吸收光谱研究了RNA和DNA（U）的稳定性，并计算了熔点。鸟嘌呤含量最高的miRNA-320a具有最高的熔点。鉴于微小RNA电化学传感器的快速发展，我们的结果将有助于研发灵敏、便携且高效的微小RNA传感器，这些传感器将能够诊断癌症和其他疾病。
