Autelitano F, Santeusanio G, Di Tondo U, Costantino A M, Renda F, Autelitano M
Cancer. 1987 Feb 1;59(3):477-83. doi: 10.1002/1097-0142(19870201)59:3<477::aid-cncr2820590321>3.0.co;2-7.
An immunohistochemical study was performed to identify the histogenesis of solid cell nests (SCN) found in 30 of 202 thyroids obtained at autopsy. Immunoperoxidase staining was used to detect the presence of calcitonin, thyroglobulin, thyroxin, low and high molecular weight keratins, and carcinoembryonic antigen (CEA). Results showed that cells forming solid nests had immunoreactivity for calcitonin, low molecular weight keratin, and CEA, but not for thyroglobulin, thyroxin and high molecular weight keratin. Thus, SCN do not result from tangentially cut thyroid follicles (absence of staining for thyroglobulin and thyroxin), nor from a squamous metaplastic process (absence of staining for high molecular weight keratin), but instead they are formed by C-cells because they showed calcitonin immunoreactivity, and neurosecretory granules.
进行了一项免疫组织化学研究，以确定在202例尸检获得的甲状腺中有30例出现的实性细胞巢（SCN）的组织发生。采用免疫过氧化物酶染色来检测降钙素、甲状腺球蛋白、甲状腺素、低分子量和高分子量角蛋白以及癌胚抗原（CEA）的存在。结果显示，形成实性巢的细胞对降钙素、低分子量角蛋白和CEA具有免疫反应性，但对甲状腺球蛋白、甲状腺素和高分子量角蛋白无免疫反应性。因此，SCN并非由甲状腺滤泡的切线切割（缺乏甲状腺球蛋白和甲状腺素染色）所致，也不是由鳞状化生过程（缺乏高分子量角蛋白染色）引起，而是由C细胞形成，因为它们显示出降钙素免疫反应性以及神经分泌颗粒。
