Institute of Biochemistry, University of Warsaw, Al. Żwirki, i Wigury 93, PL 02-089, Warszawa, Poland.
Planta. 1979 Jan;145(2):137-43. doi: 10.1007/BF00388709.
Proteins of fresh, cold and dark-stored and illuminated tomato leaves were fractionated by SDS electrophoresis. The total soluble proteins extracted from fresh leaves were separated into 5 main fractions with MWs of 54,000, 45,000, 32,000, 23,000 and 14,000. The cold and dark storage of the leaves causes a marked reduction mainly in the fraction with MW of 45,000 which increased with the illumination of the cold and dark-storaged leaves. The polypeptides with MWs of 54,000 and 14,000 (probably large and small subunits of ribulose, bisphosphate carboxylase) were stable under these conditions. In contrast, the polypeptides with MWs of 54,000 and 14,000 are decreased following the storage of tomato leaves in the dark at room temperature. Chloroplast soluble proteins were seperated by SDS electrophoresis into fractions with MWs of 64,000, 54,000, 20,000 and 14,000. The same fractions in similar proportions were observed in soluble-chloroplast proteins from fresh as well as coold and dark-stored and illuminated leaves. No drastic changes in structural polypeptides were observed following cold and dark-storage and illumination of the leaves. The results indicated that the main protein fraction, which degradated following cold and dark storage of tomato leaves and synthetized during illumination, is the fraction of cytoplasmic protein which in SDS electrophoresis gives polypeptides of about 45,000 MW. The fractions of chloroplast proteins were stable under such conditions.
采用 SDS 电泳法对新鲜、冷藏避光、照光处理的番茄叶片中的蛋白质进行了分级分离。从新鲜叶片中提取的总可溶性蛋白质被分离成 5 个主要组分，MW 分别为 54000、45000、32000、23000 和 14000。叶片的冷藏避光处理会导致 MW 为 45000 的主要组分显著减少，而这一减少量在冷避光贮藏叶片的照光处理后会增加。MW 为 54000 和 14000 的多肽（可能是核酮糖二磷酸羧化酶的大亚基和小亚基）在这些条件下稳定。相比之下，MW 为 54000 和 14000 的多肽在番茄叶片于室温黑暗条件下贮藏时会减少。SDS 电泳将叶绿体可溶性蛋白分离成 MW 为 64000、54000、20000 和 14000 的组分。在新鲜叶片以及冷藏避光和照光处理的叶片的可溶性叶绿体蛋白中，观察到了相同比例的相同组分。叶片的冷藏避光处理和照光处理后，结构多肽没有发生明显变化。结果表明，主要的蛋白质组分在番茄叶片的冷藏避光贮藏和照光处理后降解，并在照光期间合成，该组分为细胞质蛋白组分，在 SDS 电泳中给出约 45000MW 的多肽。叶绿体蛋白组分在这些条件下稳定。
