Dong Chenhui, Du Quanyin, Wang Zimin, Wang Yu, Wu Siyu, Wang Aimin
Department of Orthopedic Surgery, The Third Affiliated Hospital of Third Military Medical UniversityChongqing 400042, China; The Center of Orthopaedic Surgery of PLA, The General Hospital of Lanzhou Military CommandGansu, China.
Department of Orthopedic Surgery, The Third Affiliated Hospital of Third Military Medical University Chongqing 400042, China.
Am J Transl Res. 2016 Nov 15;8(11):4975-4981. eCollection 2016.
MicroRNAs (miRNAs) are small, short and noncoding RNAs that regulate gene expression posttranscriptionally. Increasing evidences have demonstrated that deregulated expression of miRNAs is found in osteosarcoma. In this study, we demonstrated that miR-665 was downregulated in osteosarcoma tissues compared to non-tumorous tissues. The overall survival (OS) of osteosarcoma patients with low miR-665 expression was lower than that of these patients with high miR-665 expression. Ectopic expression of miR-665 suppressed the osteosarcoma cell proliferation, EMT and invasion. We identified Rab23 as a direct target gene of miR-665. Rab23 was downregulated in osteosarcoma tissues and cell lines. The expression of miR-665 was inversely associated with the expression of Rab23 in the osteosarcoma tissues. These results suggested that miR-665 acted as a tumor suppressor gene in the development of osteosarcoma.
微小RNA（miRNA）是一类小的、短的非编码RNA，它们在转录后水平调节基因表达。越来越多的证据表明，骨肉瘤中存在miRNA表达失调的情况。在本研究中，我们发现与非肿瘤组织相比，miR-665在骨肉瘤组织中表达下调。miR-665低表达的骨肉瘤患者的总生存期（OS）低于miR-665高表达的患者。miR-665的异位表达抑制了骨肉瘤细胞的增殖、上皮-间质转化（EMT）和侵袭。我们鉴定出Rab23是miR-665的直接靶基因。Rab23在骨肉瘤组织和细胞系中表达下调。在骨肉瘤组织中，miR-665的表达与Rab23的表达呈负相关。这些结果表明，miR-665在骨肉瘤的发生发展中起着肿瘤抑制基因的作用。
