从蚕豆分生区根细胞中多聚腺苷酸化 RNA 的分离与鉴定。
Fractionation and characterization of polyadenylated RNA from broad bean meristematic root cells.
机构信息
Physiologie cellulaire végétale, Université Paris VII, 2 place Jussieu, 75005, Paris, France.
出版信息
Plant Mol Biol. 1981 Mar;1(1):53-62. doi: 10.1007/BF00023013.
Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases other characteristics of the molecule are involved. Evidence was obtained that 45-60% of the nucleotides of plant polyadenylated RNA are in base paired regions, as was previously demonstrated for mammalian mRNA.
从蚕豆分生组织根细胞的多聚腺苷酸 RNA 中，通过从聚（U）-琼脂糖分步热洗脱，然后进行顺序酚提取，分离出几个多聚腺苷酸 RNA 群体。对这些级分的分析表明，多聚（A）段的大小可能影响这种分级分离，但在某些情况下，还涉及到分子的其他特性。有证据表明，植物多聚腺苷酸 RNA 的 45-60%的核苷酸位于碱基配对区域，如以前对哺乳动物 mRNA 的研究所示。
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