Crossen P E, Durie B G, Trent J M
Cell Tissue Kinet. 1986 Sep;19(5):533-8. doi: 10.1111/j.1365-2184.1986.tb00753.x.
Generation time (GT) of normal human bone marrow cells cultured in the CFC-gm assay was measured by using bromodeoxyuridine (BrdU) incorporation and sister chromatid differential staining. Cells were cultured in methylcellulose for 72 hr prior to the addition of BrdU and then harvested at 6-12 hr intervals for up to 72 hr. The time interval between the appearance of second and third division metaphases at the 50% level gave mean GTs which ranged from 32 to 43 hr. These values are longer than those reported for myeloblasts and promyelocytes but shorter than those previously reported for myelocytes.
在集落形成细胞-粒细胞巨噬细胞（CFC-gm）检测中培养的正常人骨髓细胞的代时（GT），通过使用溴脱氧尿苷（BrdU）掺入和姐妹染色单体差异染色来测量。细胞在甲基纤维素中培养72小时，然后加入BrdU，随后每隔6 - 12小时收获一次，共收获72小时。在50%水平下，第二次和第三次分裂中期出现之间的时间间隔给出的平均代时范围为32至43小时。这些值比报道的成髓细胞和早幼粒细胞的代时长，但比先前报道的髓细胞的代时短。
