Dresch C, Troccoli G, Mary J Y
Cell Tissue Kinet. 1986 Jan;19(1):11-22. doi: 10.1111/j.1365-2184.1986.tb00711.x.
The labelling index (LI) of myelocytes (M) after flash labelling of normal human bone marrow cells with [3H]-thymidine ([3H]TdR) is always lower that the LI obtained for myeloblasts (MB) and for promyelocytes (PM). This fact can be interpreted in two ways: it may mean that the duration of the G1 phase of the cell cycle is longer in M than in MB or PM, or it may mean that the proportion of cells in cycle, i.e., the growth fraction (GF), is lower in the M population than in MB or PM. The evolution of the LI and of the mean number of grains per cell was monitored in [3H]TdR-labelled normal bone marrow during in vitro incubation for 50 hr. The generation time, measured by the halving time of the mean number of grains per cell after flash labelling, was similar for M to that for MB and PM. During continuous labelling, the LI of MB and PM reached 1 and the LI value for M never rose to more than 50% of the values recorded for MB and PM after 30 hr. These findings give support to the second hypothesis, i.e., a lower GF in the M population. Good correlation was found between the LI of M and the proportion of mature polymorphonuclear cells in the bone marrow of normal subjects and of patients with chronic benign neutropenia or hyperleucocytosis. Variations in the M growth fraction could be a medium-term (2-3 days) regulatory factor in granulocyte production.
用[3H] - 胸腺嘧啶核苷（[3H]TdR）对正常人骨髓细胞进行脉冲标记后，髓细胞（M）的标记指数（LI）始终低于原始粒细胞（MB）和早幼粒细胞（PM）的LI。这一事实可从两方面来解释：这可能意味着细胞周期G1期的持续时间在M中比在MB或PM中更长，或者这可能意味着处于细胞周期中的细胞比例，即生长分数（GF），在M群体中比在MB或PM中更低。在体外培养50小时期间，对[3H]TdR标记的正常骨髓中LI和每个细胞的平均颗粒数的变化进行了监测。通过脉冲标记后每个细胞平均颗粒数的减半时间来测量的世代时间，M与MB和PM的相似。在连续标记期间，MB和PM的LI达到1，而M的LI值在30小时后从未升至超过MB和PM记录值的50%。这些发现支持了第二种假设，即M群体中GF较低。在正常受试者以及慢性良性中性粒细胞减少症或白细胞增多症患者的骨髓中，发现M的LI与成熟多形核细胞的比例之间存在良好的相关性。M生长分数的变化可能是粒细胞生成的中期（2 - 3天）调节因子。
