Department of Biology, University of Crete, Heraklion, Crete, Greece.
Photosynth Res. 1993 Oct;38(1):83-8. doi: 10.1007/BF00015064.
The three main polyamines putrescine (Put), spermidine (Spd) and spermine (Spm) were characterized by HPLC in intact spinach leaf cells, intact chloroplasts, thylakoid membranes, Photosystem II membranes, the light-harvesting complex and the PS II complex. All contain the three polyamines in various ratios; the HPLC polyamine profiles of highly resolved PS II species (a Photosystem II core and the rection center) suggest an enrichment in the polyamine Spm.
采用 HPLC 法对完整的菠菜叶细胞、完整的叶绿体、类囊体膜、光合系统 II 膜、光捕获复合物和 PS II 复合物中三种主要的多胺腐胺(Put)、亚精胺(Spd)和精胺(Spm)进行了鉴定。这些复合物中都含有这三种多胺,且比例各不相同;高分辨率 PS II 种(一个 PS II 核心和反应中心)的 HPLC 多胺图谱表明 Spm 多胺富集。