Department of Biology, Yale University, New Haven, Connecticut 06511.
Plant Physiol. 1982 Mar;69(3):701-6. doi: 10.1104/pp.69.3.701.
A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by reverse phase HPLC, isocratic elution with methanol:water (64%, v/v) through a 5-mum C(18) column, and detection at 254 nm. The method can be directly applied to crude plant extracts, and it is not subject to interference by carbohydrates and phenolics. A good quantitative correlation was found between HPLC analysis of benzoylpolyamines and thin layer chromatography of their dansyl derivatives. With the HPLC method, polyamine titers have been reproducibly estimated for various organs of amaranth, Lemna, oat, pea, Pharbitis, and potato. The analyses correlate well with results of thin layer chromatography determinations.
已经开发出一种基于高效液相色谱法（HPLC）的衍生化方法，用于分析植物提取物中的多胺，灵敏度为 0.01-1 nmol。衍生化试剂为苯甲酰氯（Redmond 和 Tseng，1979，J Chromatogr 170:479-481）。腐胺、尸胺、胍丁胺、亚精胺、精胺和较少见的多胺正-亚精胺和同型亚精胺可以通过反相 HPLC 完全分离，使用甲醇：水（64%，v/v）等度洗脱，通过 5-μm C(18)柱，在 254nm 处检测。该方法可以直接应用于粗提植物提取物，不受碳水化合物和酚类物质的干扰。HPLC 分析苯甲酰多胺与它们的丹磺酰衍生物的薄层色谱分析之间存在良好的定量相关性。使用 HPLC 方法，可以为苋菜、浮萍、燕麦、豌豆、蕹菜和土豆的各种器官重现性地估算多胺含量。分析结果与薄层色谱测定结果密切相关。
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