Biochemisch Laboratorium, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.
Plant Mol Biol. 1982 Dec;1(4):265-76. doi: 10.1007/BF00027558.
Stable cointegrates between incRh-1 octopine (Ach5) and nopaline (C58) Ti-plasmids, present in ten independently isolated Agrobacterium tumefaciens strains, showed identical restriction endonuclease patterns. Each cointegration event had taken place in the common sequence between the T-regions of both Ti-plasmids. This illustrates a high preference for this region when used in the formation of cointegrates. Four crown gall tissues, obtained after transformation of Nicotiana tabacum cells by one of the mutants, were analysed by using Southern blot analysis for their T-DNA structure. The borders of T-DNA frequently appeared to differ from T-DNA borders previously detected in tumour tissues that had been induced by Agrobacterium strain C58 or Ach5. Therefore, it was concluded that possibly a less stringent mechanism exists for the integration into plant DNA of T-DNA, derived from a composite (octopine/nopaline) T-region than for integration of T-DNA from a normal (octopine or nopaline) T-region.
稳定的共整合物存在于十个独立分离的根癌农杆菌菌株中，整合到 Rh-1 胭脂碱（Ach5）和胭脂碱（C58）Ti 质粒的 nopaline 之间，表现出相同的限制内切酶图谱。每个共整合事件都发生在两个 Ti 质粒的 T 区之间的共同序列中。这说明了在形成共整合物时，该区域具有很高的偏好性。用其中一个突变体转化烟草细胞后获得的四个冠瘿组织，通过 Southern 印迹分析其 T-DNA 结构进行了分析。T-DNA 的边界似乎经常与以前在由农杆菌菌株 C58 或 Ach5 诱导的肿瘤组织中检测到的 T-DNA 边界不同。因此，可以得出结论，与整合来自正常（胭脂碱或胭脂碱）T 区的 T-DNA 相比，源自复合（胭脂碱/胭脂碱）T 区的 T-DNA 整合到植物 DNA 中可能存在一种不太严格的机制。
