Department of Botany, University of North Carolina, 27514, Chapel Hill, NC.
Planta. 1979 Jan;146(2):113-7. doi: 10.1007/BF00388220.
Potassium uptake in normal and crown-gall tissue culture cells of Vinca rosea L. (Cathalanthus roseus G. Don.) was studied. When grown on White's medium for 14-20 d, the tumor cells had a higher K(+) content that did the normal cells. At this logarithmic stage of growth, the rate of K(+) uptake was greater in normal cells than in tumor cells. K(+) uptake in both normal and tumor cells was inhibited substantially by 2,4-dinitrophenol but only slightly by oligomycin, NaF, and N,N'-dicyclohexyl carbodiimide. When the internal K(+) content of the cells was varied by growing the tissue in White's medium supplemented with varying concentrations of K(+), the rate of K(+) uptake in normal cells increased with decreasing internal K(+) concentrations. In contrast both lines of tumor cells failed to show this normal increase in the rate of K(+) uptake with decreasing internal K(+) concentrations.
我们研究了长春花（长春花）正常和冠瘿组织培养细胞中的钾摄取。当在 White 培养基上生长 14-20 天时，肿瘤细胞的 K(+) 含量高于正常细胞。在这个对数生长期，正常细胞的 K(+) 摄取率高于肿瘤细胞。2,4-二硝基苯酚可显著抑制正常细胞和肿瘤细胞的 K(+) 摄取，但寡霉素、NaF 和 N,N'-二环己基碳二亚胺的抑制作用较小。当通过在 White 培养基中生长组织来改变细胞内 K(+) 含量时，正常细胞的 K(+) 摄取率随内部 K(+) 浓度的降低而增加。相比之下，两条肿瘤细胞系均未能显示出随着内部 K(+) 浓度的降低，K(+) 摄取率的正常增加。
