Peterson J B, Miller C O
Department of Plant Sciences, Indiana University, Bloomington, Indiana 47401.
Plant Physiol. 1976 Mar;57(3):393-9. doi: 10.1104/pp.57.3.393.
Several compounds containing reduced nitrogen markedly increased the yields of cell-division compounds extractable from an A6 Vinca rosea L. crown gall tumor tissue. Casein hydrolysate, several amino acids, and ammonium salts were effective. Both trans-zeatin and ribosyl-trans-zeatin were substantially increased in total amount per culture and in concentration. These two compounds have been identified by several criteria including mass spectra. The reduced nitrogen treatments also caused the appearance of a cytokinin not previously detected in this tissue; it has not yet been identified. The tumor tissue rapidly absorbed [8-(14)C]adenine from a liquid medium. Within 1 hour, the tissue converted some of the adenine to zeatin and ribosylzeatin, and greater degrees of conversion occurred in 2-, 4-, and 8-hour periods. The tissue grown on a medium containing ammonium chloride accumulated considerably greater quantities of the two cytokinins made from the labeled adenine during each incubation period.
几种含还原态氮的化合物显著提高了从长春花A6冠瘿瘤组织中可提取的细胞分裂化合物的产量。酪蛋白水解物、几种氨基酸和铵盐都有效果。反式玉米素和核糖基反式玉米素的总量和浓度均大幅增加。这两种化合物已通过包括质谱在内的多种标准进行了鉴定。还原态氮处理还导致该组织中出现了一种以前未检测到的细胞分裂素；目前尚未鉴定出来。肿瘤组织能迅速从液体培养基中吸收[8-(14)C]腺嘌呤。在1小时内，该组织将部分腺嘌呤转化为玉米素和核糖基玉米素，在2小时、4小时和8小时期间转化率更高。在含有氯化铵的培养基上生长的组织在每个培养期积累的由标记腺嘌呤合成的两种细胞分裂素的量要多得多。
