诱导和分离烟草体细胞培养物中的营养缺陷型突变体。
Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum.
出版信息
Science. 1970 Apr 24;168(3930):487-9. doi: 10.1126/science.168.3930.487.
PMID:17838129
Abstract
A method for the selection of auxotrophic mutants from somatic cell cultures of Nicotiana tabacum depends upon the incorporation of 5-bromodeoxyuridine into the DNA of wild-type cells and upon its lack of incorporation into the DNA of auxotrophic cells. There may be more than one functional copy of essential genes in the haploid genome of Nicotiana tabacum.
摘要
一种从烟草体细胞培养物中选择营养缺陷型突变体的方法，依赖于将 5-溴脱氧尿苷掺入野生型细胞的 DNA 中，以及依赖于它不能掺入营养缺陷型细胞的 DNA 中。在烟草的单倍体基因组中，可能有一个以上的必需基因的功能拷贝。
Zotero 插件