Department of Developmental Plant Biology, Biological Centre, State University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands.
Plant Mol Biol. 1983 Nov;2(6):295-303. doi: 10.1007/BF01578590.
The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitro translation product of Mr27 500 and isoelectric point of 4.7.
豌豆幼苗 mRNA 序列编码一种芽特异性多肽的 cDNA 分子克隆，该多肽是核酮糖 1,5-二磷酸羧化酶的小亚基，也是光捕获叶绿素 a/b 复合物的一个组成部分。从小麦幼苗多聚核糖体 poly(A)RNA 中制备的 cDNA 通过与黑暗生长的根的巯基化多聚核糖体 poly(A)RNA 进行异源液相杂交，并用硫醇色谱法进行富集，得到了芽特异性和光诱导序列。通过杂交释放翻译产物的 2D 电泳分析鉴定了克隆的芽特异性序列。克隆的芽特异性序列与一个在光和黑暗生长的芽中都存在的 850nt 的 mRNA 相对应，体外翻译产物的分子量为 27500，等电点为 4.7。
