Department of Developmental Plant Biology, Biological Centre, State University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands.
Plant Mol Biol. 1985 Mar;4(2-3):95-102. doi: 10.1007/BF02418755.
The regulation of a mRNA encoding a shoot-specific polypeptide from developing pea seedlings was studied and compared to the regulation of mRNAs encoding two major light-induced nuclear-encoded polypeptides, the small subunit of the ribulose 1,5 biphosphate carboxylase (ssRuBPCase) and a polypeptide of the light-harvesting chlorophyll a/b complex (LHCP). By using cDNA clones as probes in Northern blottings of total cellular RNA it was found that both ssRuBPCase and LHCP mRNA could be induced in shoots by white and red light but to lower levels in roots and cotyledons. In contrast, the mRNA for the shoot-specific polypeptide was only found in shoots, and was present approximately two days after the start of germination. The shoot-specific mRNA sequence was predominantly found in stem tissue, irrespective of illumination, both in the young seedlings and adult plants. Only very low amounts could be detected in plumule and leaf. The shoot-specific sequence could also be detected in RNA isolated from developing shoots of another pea cultivar but not in those of other legumes and of cereals. The primary sequence of the complete coding portion and the deduced amino acid sequence of the mRNA encoding the shoot-specific polypeptide was determined. The observed codon usage is non-random and is consistent with data from other high plant genes. Possible polyadenylation signal sequences (AATAAG and AATAAT) were present at 55 and 124 bases 5' of the poly(A) tail. The polypeptide encoded by the shoot-specific mRNA consists of 196 amino acids with a calculated molecular weight of 21 898. It contains a four times reiterated highly conserved unit of 26 amino acids. The NH2-terminal end is highly hydrophobic and resembles a signal polypeptide.
从发育中的豌豆幼苗中分离出一种编码茎特异性多肽的 mRNA 的调控进行了研究，并与编码两种主要的光诱导核编码多肽的 mRNA 的调控进行了比较，这两种多肽是核酮糖 1,5-二磷酸羧化酶的小亚基（ssRuBPCase）和一种光捕获叶绿素 a/b 复合物（LHCP）的多肽。通过使用 cDNA 克隆作为总细胞 RNA 的 Northern 印迹中的探针，发现 ssRuBPCase 和 LHCP mRNA 均可被白光和红光诱导，但在根和子叶中的诱导水平较低。相比之下，编码茎特异性多肽的 mRNA 仅在茎中发现，并且在萌发开始后约两天出现。该茎特异性 mRNA 序列主要存在于茎组织中，无论光照如何，在幼苗和成年植物中均如此。仅在芽和叶中检测到极低的含量。该茎特异性序列也可以从另一种豌豆品种发育中的茎中分离的 RNA 中检测到，但不能从其他豆科植物和谷类植物中检测到。确定了编码茎特异性多肽的 mRNA 的完整编码部分的序列和推导的氨基酸序列。观察到的密码子使用是非随机的，与其他高等植物基因的数据一致。在 poly(A) 尾的 5'端存在 55 个和 124 个碱基的可能多聚腺苷酸化信号序列（AATAAG 和 AATAAT）。由茎特异性 mRNA 编码的多肽由 196 个氨基酸组成，计算分子量为 21898。它包含一个四次重复的高度保守的 26 个氨基酸单元。NH2-末端高度疏水性，类似于信号多肽。
