Cohn N S, Mitchell J P
Histochemistry. 1986;84(4-6):432-8. doi: 10.1007/BF00482975.
Although there may be documented morphological changes during development, it is obvious that important changes in protein content occur in a vascular plant during the several stages of differentiation. In the absence of the latter information, an approach has been established for the localization of antigenic proteins in developing tissues of Pisum sativum. Monoclonal antibodies were raised to proteins extracted from pea internode tissue, and employed for the localization of three proteins in tissue sections. One of the proteins has two polypeptide subunits with molecular weights of 25,000 and 40,000 daltons, and the antibody binds to both of them. The three monoclonal antibodies produce different patterns of cellular localization in the tissue sections, as visualized by indirect immunocytochemical labeling. In another series of analyses, quantitative and qualitative differences in the protein contents of apical shoot tissue and mature internode shoot tissue have been found. These studies were based on the use of Western blots with both polyclonal (rabbit) antibodies and monoclonal (mouse) antibodies.
虽然在发育过程中可能有形态变化的记录，但显然在维管植物分化的几个阶段中，蛋白质含量会发生重要变化。在缺乏后者信息的情况下，已经建立了一种在豌豆发育组织中定位抗原蛋白的方法。针对从豌豆节间组织中提取的蛋白质制备了单克隆抗体，并用于在组织切片中定位三种蛋白质。其中一种蛋白质有两个分子量分别为25,000和40,000道尔顿的多肽亚基，抗体与两者都结合。通过间接免疫细胞化学标记可视化，这三种单克隆抗体在组织切片中产生不同的细胞定位模式。在另一系列分析中，发现顶芽组织和成熟节间芽组织的蛋白质含量存在定量和定性差异。这些研究基于使用多克隆（兔）抗体和单克隆（鼠）抗体的蛋白质印迹法。
