Centre d'Etude de l'Hérédité Cytoplasmique, Laboratoire d'Enzymologie., Université Catholique de Louvain, Place Croix du Sud, 1, B-1348, Louvain-la-Neuve, Belgium.
Plant Mol Biol. 1983 Nov;2(6):305-9. doi: 10.1007/BF01578591.
Biochemical analysis and electron microscopy showed that mitochondria of both the fertile and the male sterile 350 and 447 cytoplasms ofVicia faba. L. contain two small supercoiled DNA molecules of mean length of 1 700 and 1 420 base pairs in addition to the main mitochondrial DNA of high molecular weight. By agarose gel electrophoresis, the male sterile cytoplasm 350 is distinguished from the fertile cytoplasm and from the male sterile cytoplasm 447 by the presence of an additional supercoiled DNA molecule of approximately 1 540 bp.
生化分析和电子显微镜观察表明,豌豆的可育和不育 350 及 447 细胞质线粒体除了具有高分子量的主要线粒体 DNA 之外,还含有两个平均长度为 1700 和 1420 个碱基对的小超螺旋 DNA 分子。通过琼脂糖凝胶电泳,不育细胞质 350 可以与可育细胞质和不育细胞质 447 区分开来,因为前者存在一个大约 1540bp 的额外超螺旋 DNA 分子。